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Association of HIV-1 Envelope-Specific Breast Milk IgA Responses with Reduced Risk of Postnatal Mother-to-Child Transmission of HIV-1
Journal article   Open access   Peer reviewed

Association of HIV-1 Envelope-Specific Breast Milk IgA Responses with Reduced Risk of Postnatal Mother-to-Child Transmission of HIV-1

Justin Pollara, Erin McGuire, Genevieve G Fouda, Wes Rountree, Josh Eudailey, R Glenn Overman, Kelly E Seaton, Aaron Deal, R Whitney Edwards, Gerald Tegha, …
Journal of virology, Vol.89(19), pp.9952-9961
10/01/2015
DOI: 10.1128/JVI.01560-15
PMCID: PMC4577885
PMID: 26202232
url
https://doi.org/10.1128/JVI.01560-15View
Published (Version of record) Open Access

Abstract

Infants born to HIV-1-infected mothers in resource-limited areas where replacement feeding is unsafe and impractical are repeatedly exposed to HIV-1 throughout breastfeeding. Despite this, the majority of infants do not contract HIV-1 postnatally, even in the absence of maternal antiretroviral therapy. This suggests that immune factors in breast milk of HIV-1-infected mothers help to limit vertical transmission. We compared the HIV-1 envelope-specific breast milk and plasma antibody responses of clade C HIV-1-infected postnatally transmitting and nontransmitting mothers in the control arm of the Malawi-based Breastfeeding Antiretrovirals and Nutrition Study using multivariable logistic regression modeling. We found no association between milk or plasma neutralization activity, antibody-dependent cell-mediated cytotoxicity, or HIV-1 envelope-specific IgG responses and postnatal transmission risk. While the envelope-specific breast milk and plasma IgA responses also did not reach significance in predicting postnatal transmission risk in the primary model after correction for multiple comparisons, subsequent exploratory analysis using two distinct assay methodologies demonstrated that the magnitudes of breast milk total and secretory IgA responses against a consensus HIV-1 envelope gp140 (B.con env03) were associated with reduced postnatal transmission risk. These results suggest a protective role for mucosal HIV-1 envelope-specific IgA responses in the context of postnatal virus transmission. This finding supports further investigations into the mechanisms by which mucosal IgA reduces risk of HIV-1 transmission via breast milk and into immune interventions aimed at enhancing this response. Infants born to HIV-1-infected mothers are repeatedly exposed to the virus in breast milk. Remarkably, the transmission rate is low, suggesting that immune factors in the breast milk of HIV-1-infected mothers help to limit transmission. We compared the antibody responses in plasma and breast milk of HIV-1-transmitting and -nontransmitting mothers to identify responses that correlated with reduced risk of postnatal HIV-1 transmission. We found that neither plasma nor breast milk IgG antibody responses were associated with risk of HIV-1 transmission. In contrast, the magnitudes of the breast milk IgA and secretory IgA responses against HIV-1 envelope proteins were associated with reduced risk of postnatal HIV-1 transmission. The results of this study support further investigations of the mechanisms by which mucosal IgA may reduce the risk of HIV-1 transmission via breastfeeding and the development of strategies to enhance milk envelope-specific IgA responses to reduce mother-to-child HIV transmission and promote an HIV-free generation.
 Adult Antibodies, Neutralizing - metabolism Antibody Specificity Antibody-Dependent Cell Cytotoxicity Breast Feeding - adverse effects env Gene Products, Human Immunodeficiency Virus - immunology Female HIV Antibodies - blood HIV Antibodies - metabolism HIV Infections - complications HIV Infections - immunology HIV Infections - transmission HIV-1 - immunology Humans Immunity, Mucosal Immunoglobulin A - blood Immunoglobulin A - metabolism Immunoglobulin A, Secretory - metabolism Immunoglobulin G - metabolism Infant Infant, Newborn Infectious Disease Transmission, Vertical Malawi Milk, Human - immunology Milk, Human - virology Models, Immunological Pregnancy Pregnancy Complications, Infectious - immunology Pregnancy Complications, Infectious - virology Risk Factors Young Adult

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