Journal article
Association of HIV diversity and survival in HIV-infected Ugandan infants
PloS one, Vol.6(4), pp.e18642-e18642
04/14/2011
DOI: 10.1371/journal.pone.0018642
PMCID: PMC3077388
PMID: 21533179
Abstract
The level of viral diversity in an HIV-infected individual can change during the course of HIV infection, reflecting mutagenesis during viral replication and selection of viral variants by immune and other selective pressures. Differences in the level of viral diversity in HIV-infected infants may reflect differences in viral dynamics, immune responses, or other factors that may also influence HIV disease progression. We used a novel high resolution melting (HRM) assay to measure HIV diversity in Ugandan infants and examined the relationship between diversity and survival through 5 years of age.
Plasma samples were obtained from 31 HIV-infected infants (HIVNET 012 trial). The HRM assay was used to measure diversity in two regions in the gag gene (Gag1 and Gag2) and one region in the pol gene (Pol).
HRM scores in all three regions increased with age from 6-8 weeks to 12-18 months (for Gag1: P = 0.005; for Gag2: P = 0.006; for Pol: P = 0.016). Higher HRM scores at 6-8 weeks of age (scores above the 75(th) percentile) were associated with an increased risk of death by 5 years of age (for Pol: P = 0.005; for Gag1/Gag2 (mean of two scores): P = 0.003; for Gag1/Gag2/Pol (mean of three scores): P = 0.002). We did not find an association between HRM scores and other clinical and laboratory variables.
Genetic diversity in HIV gag and pol measured using the HRM assay was typically low near birth and increased over time. Higher HIV diversity in these regions at 6-8 weeks of age was associated with a significantly increased risk of death by 5 years of age.
Details
- Title: Subtitle
- Association of HIV diversity and survival in HIV-infected Ugandan infants
- Creators
- Maria M James - Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of AmericaLei WangPhilippa MusokeDeborah DonnellJessica FogelWilliam I TowlerLeila KhakiClemensia NakabiitoJ Brooks JacksonSusan H Eshleman
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.6(4), pp.e18642-e18642
- DOI
- 10.1371/journal.pone.0018642
- PMID
- 21533179
- PMCID
- PMC3077388
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library of Science; United States
- Grant note
- U01-AI068613 / NIAID NIH HHS U01 AI068613 / NIAID NIH HHS N01 AI045200 / NIAID NIH HHS U01 AI068617 / NIAID NIH HHS N01-AI035173 / NIAID NIH HHS N01-AI045200 / NIAID NIH HHS U01-AI068632 / NIAID NIH HHS U01 AI068632 / NIAID NIH HHS R01 AI087139 / NIAID NIH HHS N0I-AI035173-417 / NIAID NIH HHS U01 AI068613-05 / NIAID NIH HHS N01 AI035173 / NIAID NIH HHS
- Language
- English
- Date published
- 04/14/2011
- Academic Unit
- Pathology; VPMA - Administration
- Record Identifier
- 9984047715702771
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