Journal article
Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells
Proceedings of the National Academy of Sciences - PNAS, Vol.81(2), pp.419-423
01/1984
DOI: 10.1073/pnas.81.2.419
PMCID: PMC344688
PMID: 6198648
Abstract
The relationship between the rat tumor-associated cytokeratin p39 (Mr 39,000) and cellular DNA has been studied in intact cells. Using a DNA-protein crosslinking technique, incubation with cis-dichlorodiammineplatinum, we present evidence for the association of p39 with DNA in intact Novikoff ascites hepatoma cells. The cells were treated with cis-dichlorodiammineplatinum and solubilized in NaDodSO4-containing buffer, and the DNA was pelleted by high-speed centrifugation. By immunotransfer analysis, the cytokeratin was found in the DNA pellet of the crosslinked samples while absent from the controls. This result was further substantiated by CsCl density-gradient centrifugation. Collectively, these results suggest a cytokeratin-DNA association at the filament binding sites on or near the nuclear lamina.
Details
- Title: Subtitle
- Association of cytokeratin p39 with DNA in intact Novikoff hepatoma cells
- Creators
- W S WardW N SchmidtC A SchmidtL S Hnilica
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.81(2), pp.419-423
- DOI
- 10.1073/pnas.81.2.419
- PMID
- 6198648
- PMCID
- PMC344688
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Grant note
- CA 26412 / NCI NIH HHS
- Language
- English
- Date published
- 01/1984
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094775902771
Metrics
20 Record Views