Journal article
Association of estrogen receptor beta polymorphisms with posterior tibial tendon dysfunction
Molecular and cellular biochemistry, Vol.471(1-2), pp.63-69
08/01/2020
DOI: 10.1007/s11010-020-03765-z
PMID: 32472323
Abstract
Posterior tibial tendon (PTT) dysfunction is three times more common in females, and some patients may have a predisposition without a clinically evident cause, suggesting that individual characteristics play an important role in tendinopathy. The present study investigated the association of rs4986938 (+ 1730G > A; AluI RFLP) and rs1256049 (- 1082G > A; RsaI RFLP) single nucleotide polymorphisms (SNPs) of estrogen receptor-beta (ER-beta) gene with PTT dysfunction. A total of 400 participants were recruited. The PTT dysfunction group: these patients underwent surgery, with PTT tendinopathy confirmed by histopathology and magnetic resonance image (MRI). The control group was composed of participants with no clinical or MRI evidence of PTT dysfunction. Each group was composed of 100 postmenopausal women, 50 premenopausal women, and 50 men. Genomic DNA was extracted from saliva samples, and genotypes were obtained by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). Concerning theER-beta SNP rs4986938, there were significant differences in the frequencies of alleles between test and control groups of all the cases, only postmenopausal women and only men (p < 0.0001,p = 0.0016 andp = 0.0001). Considering the PTT dysfunction group and comparing postmenopausal women versus premenopausal women adding men, the analysis showed significant differences in the allelic distribution (p = 0.0450): the allele A in postmenopausal women is a risk factor. TheER-beta SNP rs1256049 did not show differences in the frequencies of alleles and genotypes between groups. TheER-beta SNP rs4986938, but not ER -beta SNPs rs1256049, may contribute to PTT insufficiency in the Brazilian population, with additional risk in postmenopausal women. Addition, in men the genetic factor could be more determinant.
Details
- Title: Subtitle
- Association of estrogen receptor beta polymorphisms with posterior tibial tendon dysfunction
- Creators
- P. R. B. Nogara - Federal University of ParanáA. L. Godoy-Santos - Universidade de São PauloF. C. P. Fonseca - Universidade de São PauloC. Cesar-Netto - MedStar Union Memorial HospitalK. C. Carvalho - Universidade de São PauloE. C. Baracat - Universidade de São PauloN. Maffulli - Queen Mary University of LondonP. A. Pontin - Universidade de São PauloM. C. L. Santos - Univ Fed Parana, Dept Cell Biol, Curitiba, Parana, Brazil
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biochemistry, Vol.471(1-2), pp.63-69
- Publisher
- Springer Nature
- DOI
- 10.1007/s11010-020-03765-z
- PMID
- 32472323
- ISSN
- 0300-8177
- eISSN
- 1573-4919
- Number of pages
- 7
- Grant note
- 400001016/007p8 / Capes; Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Language
- English
- Date published
- 08/01/2020
- Academic Unit
- Orthopedics and Rehabilitation
- Record Identifier
- 9984304689502771
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