Association of interleukin-1beta and interleukin-1 receptor antagonist polymorphisms with bacterial vaginosis in non-pregnant Italian women

Sabina Cauci; Manuela Di Santolo; Giorgia Casabellata; Kelli Ryckman; Scott M Williams; Secondo Guaschino

doi:10.1093/molehr/gam002

Back

Association of interleukin-1beta and interleukin-1 receptor antagonist polymorphisms with bacterial vaginosis in non-pregnant Italian women

Journal article

Peer reviewed

Association of interleukin-1beta and interleukin-1 receptor antagonist polymorphisms with bacterial vaginosis in non-pregnant Italian women

Sabina Cauci, Manuela Di Santolo, Giorgia Casabellata, Kelli Ryckman, Scott M Williams and Secondo Guaschino

Molecular human reproduction, Vol.13(4), pp.243-250

04/2007

DOI: 10.1093/molehr/gam002

PMID: 17314118

Files and links (1)

url

http://molehr.oxfordjournals.org/content/13/4/243.longView

Open Access

Abstract

Bacterial vaginosis (BV) is the most prevalent alteration of vaginal microflora worldwide. BV is a polymicrobial disorder, and its etiology is elusive. Factors predisposing to this recurrent condition are not fully characterized. We aimed to investigate whether interleukin-1beta (IL-1beta) and IL-1 receptor antagonist (IL-1ra) polymorphisms are associated with BV in non-pregnant white Italian women. Genomic DNA was obtained from 164 BV positive, and 406 control women. Two diallelic polymorphisms in the IL-1beta gene (IL-1B) representing C/T base transitions at - 511 and + 3954 positions and a variable number tandem repeats (VNTR) in intron 2 of the IL-1ra gene (IL-1RN) were assessed. We demonstrated that women who were homozygous for - 511 CC or + 3954 TT of the IL-1B gene were at increased risk for BV with an odds ratio (OR) = 1.5 [95% confidence interval (CI) = 1.03-2.14, P = 0.032], and OR = 2.8 (95% CI = 1.37-5.88, P = 0.004), respectively. The haplotype - 511/ + 3954 T-C was protective for BV, with an OR = 0.7 (95% CI = 0.49-0.90, P = 0.009). The IL-1RN VNTR genotype was not associated with BV, although the rare allele 3 showed a trend towards protection (P = 0.049). These data show that host genetic variants at the IL-1beta locus predispose to BV among Caucasian non-pregnant women. Further studies will determine whether these genetic polymorphisms modulate the risk for BV recurrence, and/or BV associated severe adverse outcomes as preterm birth and human immunodeficiency virus transmission.

Italy

Haplotypes

European Continental Ancestry Group - genetics

Genetic Predisposition to Disease

Risk Assessment

Introns

Vaginosis, Bacterial - genetics

Gene Frequency

Humans

Middle Aged

Risk Factors

Interleukin 1 Receptor Antagonist Protein - genetics

Interleukin-1beta - genetics

Case-Control Studies

Homozygote

Vaginosis, Bacterial - microbiology

Adult

Female

Polymorphism, Single Nucleotide

Odds Ratio

Peptide Fragments - genetics

Details

Title: Subtitle: Association of interleukin-1beta and interleukin-1 receptor antagonist polymorphisms with bacterial vaginosis in non-pregnant Italian women
Creators: Sabina Cauci - Department of Biomedical Sciences and Technologies, School of Medicine, University of Udine, Udine, Italy. scauci@mail.dstb.uniud.it
Manuela Di Santolo
Giorgia Casabellata
Kelli Ryckman
Scott M Williams
Secondo Guaschino
Resource Type: Journal article
Publication Details: Molecular human reproduction, Vol.13(4), pp.243-250
DOI: 10.1093/molehr/gam002
PMID: 17314118
NLM abbreviation: Mol Hum Reprod
ISSN: 1360-9947
eISSN: 1460-2407
Publisher: England
Language: English
Date published: 04/2007
Academic Unit: Stead Family Department of Pediatrics; Epidemiology
Record Identifier: 9983995019802771

Metrics

38 Record Views

20 Times Cited - Web of Science