Journal article
Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
PloS one, Vol.11(7), pp.e0159756-e0159756
07/25/2016
DOI: 10.1371/journal.pone.0159756
PMCID: PMC4959720
PMID: 27454313
Abstract
Background
Ossification of the posterior longitudinal ligament (OPLL) of the spine is considered a multifactorial and polygenic disease. We aimed to investigate the association between four single nucleotide polymorphisms (SNPs) of pre-miRNAs [miR-146aC>G (rs2910164), miR149T>C (rs2292832), miR-196a2T>C (rs11614913), and miR-499A>G (rs3746444)] and the risk of cervical OPLL in the Korean population.
Methods
The genotypic frequencies of these four SNPs were analyzed in 207 OPLL patients and 200 controls by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay.
Findings
For four SNPs in pre-miRNAs, no significant differences were found between OPLL patients and controls. However, subgroup analysis based on OPLL subgroup (continuous: continuous type plus mixed type, segmental: segmental and localized type) showed that miR-499GG genotype was associated with an increased risk of segmental type OPLL (adjusted odds ratio = 4.314 with 95% confidence interval: 1.109-16.78). In addition, some allele combinations (C-T-T-G, G-T-T-A, and G-T-C-G of miR-146a/-149/-196a2/-499) and combined genotypes (miR-149TC/miR-196a2TT) were associated with increased OPLL risk, whereas the G-T-T-G and G-C-C-G allele combinations were associated with decreased OPLL risk.
Conclusion
The results indicate that GG genotype of miR-499 is associated with significantly higher risks of OPLL in the segmental OPLL group. The miR-146a/-149/-196a2/-499 allele combinations may be a genetic risk factor for cervical OPLL in the Korean population.
Details
- Title: Subtitle
- Association of miR-146a, miR-149, miR-196a2, and miR-499 Polymorphisms with Ossification of the Posterior Longitudinal Ligament of the Cervical Spine
- Creators
- Jae Joon Lim - CHA University Bundang Medical CenterDong Ah Shin - Severance HospitalYoung Joo Jeon - Green Cross (South Korea)Hemant Kumar - CHA University Bundang Medical CenterSeil Sohn - CHA University Bundang Medical CenterHyoung Sik Min - Korea Univ, Dept Neurosurg, Coll Med, Seoul, South KoreaJang Bo Lee - Korea UniversitySung Uk Kuh - Gangnam Severance HospitalKeung Nyun Kim - Yonsei UniversityJung Oh Kim - CHA UniversityOk Joon Kim - CHA UniversityAlexander E. Ropper - Baylor College of MedicineNam Keun Kim - CHA University Bundang Medical CenterIn Bo Han - CHA University
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.11(7), pp.e0159756-e0159756
- DOI
- 10.1371/journal.pone.0159756
- PMID
- 27454313
- PMCID
- PMC4959720
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 14
- Grant note
- NRF-2014R1A1A2059118 / National Research Foundation of Korea (NRF); National Research Foundation of Korea H114C3245 / Korea Health Technology Research and Development Project, Ministry for Health and Welfare Affairs
- Language
- English
- Date published
- 07/25/2016
- Academic Unit
- Neurosurgery
- Record Identifier
- 9984460325502771
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