Logo image
Association of the Skn7 and Yap1 transcription factors in the Saccharomyces cerevisiae oxidative stress response
Journal article   Open access   Peer reviewed

Association of the Skn7 and Yap1 transcription factors in the Saccharomyces cerevisiae oxidative stress response

K E Mulford and J S Fassler
Eukaryotic cell, Vol.10(6), pp.761-769
06/2011
DOI: 10.1128/EC.00328-10
PMCID: PMC3127664
PMID: 21478431
url
https://doi.org/10.1128/EC.00328-10View
Published (Version of record) Open Access

Abstract

Saccharomyces cerevisiae Skn7p is a stress response transcription factor that undergoes aspartyl phosphorylation by the Sln1p histidine kinase. Aspartyl phosphorylation of Skn7p is required for activation of genes required in response to wall stress, but Skn7p also activates oxidative stress response genes in an aspartyl phosphorylation-independent manner. The presence of binding sites for the Yap1p and Skn7p transcription factors in oxidative stress response promoters and the oxidative stress-sensitive phenotypes of SKN7 and YAP1 mutants suggest that these two factors work together. We present here evidence for a DNA-independent interaction between the Skn7 and Yap1 proteins that involves the receiver domain of Skn7p and the cysteine-rich domains of Yap1p. The interaction with Yap1p may help partition the Skn7 protein to oxidative stress response promoters when the Yap1 protein accumulates in the nucleus.
Protein Structure, Tertiary Peptide Fragments - metabolism Saccharomyces cerevisiae - physiology Immunoprecipitation Response Elements Transcriptional Activation Oxidative Stress - genetics Mutation, Missense Saccharomyces cerevisiae Proteins - genetics Transcription Factors - genetics DNA-Binding Proteins - genetics Gene Knockout Techniques DNA-Binding Proteins - metabolism Saccharomyces cerevisiae - metabolism Transcription Factors - metabolism Phenotype Saccharomyces cerevisiae Proteins - metabolism Protein Binding Genes, Fungal Genes, Reporter

Details

Metrics

Logo image