Journal article
Associations of genetics and sampling time with levels of interleukin-2 activity
Veterinary immunology and immunopathology, Vol.22(4), pp.333-343
11/15/1989
DOI: 10.1016/0165-2427(89)90169-4
PMID: 2560593
Abstract
The interleukin-2 (IL-2) assay has become a useful tool for examining the cellular immune response. Because of a lack of avian IL-2-dependent cell lines, the avian IL-2 assay in its present state, however, is not currently as powerful as its mammalian counterpart in effectively evaluating levels of IL-2 activity. The use of an avian IL-2 reference standard, Percoll-enriched populations of lymphoblasts, and sample collection times were examined to optimize the assay for comparing levels of IL-2 activity in a large number of birds. The reference standard was effective in accounting for assay-to-assay variance. Percoll-enriched populations of responding lymphoblasts were more sensitive to IL-2 than nonseparated populations of cultured peripheral blood lymphocytes. Levels of IL-2 activity of cells isolated from the same bird did not change within a 1-week period, although differences did approach significance. In addition, levels of IL-2 activity differed between genetically distinct populations.
Details
- Title: Subtitle
- Associations of genetics and sampling time with levels of interleukin-2 activity
- Creators
- K L Knudtson - Iowa State UniversityS J Lamont - Iowa State University
- Resource Type
- Journal article
- Publication Details
- Veterinary immunology and immunopathology, Vol.22(4), pp.333-343
- DOI
- 10.1016/0165-2427(89)90169-4
- PMID
- 2560593
- ISSN
- 0165-2427
- eISSN
- 1873-2534
- Language
- English
- Date published
- 11/15/1989
- Academic Unit
- Iowa Institute of Human Genetics
- Record Identifier
- 9984627229102771
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