Journal article
Astrocyte-derived lipoxygenase product evokes endothelium-dependent relaxation of the basilar artery
Journal of neuroscience research, Vol.38(3), pp.314-318
06/15/1994
DOI: 10.1002/jnr.490380309
PMID: 7523688
Abstract
The goal of this study was to examine the possible production of vasoactive factors by astrocytes. We consistently observe that rat astroglial cells in suspension produce marked relaxation when added to precontracted rings of intact (but not endothelium-denuded) rabbit basilar artery. The ultimate mediator of this relaxation was endothelium-derived nitric oxide whose synthesis is activated by an as yet unidentified factor(s) produced tonically by astrocytes. The factor is relatively stable, and is not arachidonate, or a product of cyclooxygenase or P450 metabolism. Based upon studies with selective inhibitors, the factor appears to result from 12- or 15-lipoxygenase metabolism, the products of which are known to be vasoactive. In a separate series of experiments, astrocyte-conditioned medium stimulated the production of citrulline from L-arginine by nitric oxide synthase in bovine aortic endothelial cells. The possible significance for central nervous system (CNS) pathophysiology of an astrocyte-derived vasodilator is discussed.
Details
- Title: Subtitle
- Astrocyte-derived lipoxygenase product evokes endothelium-dependent relaxation of the basilar artery
- Creators
- S Murphy - Department of Pharmacology, University of Iowa College of Medicine, Iowa City 52242G RichK I OrgrenS A MooreF M Faraci
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience research, Vol.38(3), pp.314-318
- Publisher
- United States
- DOI
- 10.1002/jnr.490380309
- PMID
- 7523688
- ISSN
- 0360-4012
- eISSN
- 1097-4547
- Grant note
- NS 24621 / NINDS NIH HHS
- Language
- English
- Date published
- 06/15/1994
- Academic Unit
- Pathology; Cardiovascular Medicine; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040315302771
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