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Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology
Journal article   Open access   Peer reviewed

Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology

Stephan A Pless, Robin Y Kim, Christopher A Ahern and Harley T Kurata
The Journal of physiology, Vol.593(12), pp.2627-2634
06/15/2015
DOI: 10.1113/jphysiol.2014.287714
PMCID: PMC4500348
PMID: 25640301
url
https://doi.org/10.1113/jphysiol.2014.287714View
Published (Version of record) Open Access

Abstract

Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.
Mutagenesis Ion Channels - physiology Animals Amino Acids - genetics Ion Channels - chemistry

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