Journal article
Atypical Cadherins Celsr1-3 Differentially Regulate Migration of Facial Branchiomotor Neurons in Mice
The Journal of neuroscience, Vol.30(28), pp.9392-9401
07/14/2010
DOI: 10.1523/JNEUROSCI.0124-10.2010
PMCID: PMC3069688
PMID: 20631168
Abstract
During hindbrain development, facial branchiomotor neurons (FBM neurons) migrate from medial rhombomere (r) 4 to lateral r6. In zebrafish, mutations in planar cell polarity genes
celsr2
and
frizzled3a
block caudal migration of FBM neurons. Here, we investigated the role of cadherins
Celsr1-3
, and
Fzd3
in FBM neuron migration in mice. In
Celsr1
mutants (knock-out and
Crash
alleles), caudal migration was compromised and neurons often migrated rostrally into r2 and r3, as well as laterally. These phenotypes were not caused by defects in hindbrain patterning or neuronal specification. Celsr1 is expressed in FBM neuron precursors and the floor plate, but not in FBM neurons. Consistent with this, conditional inactivation showed that the function of Celsr1 in FBM neuron migration was non-cell autonomous. In
Celsr2
mutants, FBM neurons initiated caudal migration but moved prematurely into lateral r4 and r5. This phenotype was enhanced by inactivation of
Celsr3
in FBM neurons and mimicked by inactivation of
Fzd3
. Furthermore,
Celsr2
was epistatic to
Celsr1
. These data indicate that
Celsr1-3
differentially regulate FBM neuron migration.
Celsr1
helps to specify the direction of FBM neuron migration, whereas
Celsr2
and
3
control its ability to migrate.
Details
- Title: Subtitle
- Atypical Cadherins Celsr1-3 Differentially Regulate Migration of Facial Branchiomotor Neurons in Mice
- Creators
- Yibo Qu - Institute of Neuroscience, Developmental Neurobiology, Université Catholique de Louvain, B1200 Brussels, BelgiumDerrick M Glasco - Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Libing Zhou - Institute of Neuroscience, Developmental Neurobiology, Université Catholique de Louvain, B1200 Brussels, BelgiumAnagha Sawant - Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Aurélia Ravni - Institute of Neuroscience, Developmental Neurobiology, Université Catholique de Louvain, B1200 Brussels, BelgiumBernd Fritzsch - Department of Biology, University of Iowa, Iowa City, Iowa 52242Christine Damrau - Mammalian Genetics Unit, Medical Research Council, Harwell, OX11 0RD, United KingdomJennifer N Murdoch - Mammalian Genetics Unit, Medical Research Council, Harwell, OX11 0RD, United KingdomSylvia Evans - Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California 92093Samuel L Pfaff - Gene Expression Laboratory, The Salk Institute, La Jolla, California 92037, andCaroline Formstone - Medical Research Council Centre for Developmental Neurobiology, Kings College London, London SE1 1UL, United KingdomAndré M Goffinet - Institute of Neuroscience, Developmental Neurobiology, Université Catholique de Louvain, B1200 Brussels, BelgiumAnand Chandrasekhar - Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211Fadel Tissir - Institute of Neuroscience, Developmental Neurobiology, Université Catholique de Louvain, B1200 Brussels, Belgium
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.30(28), pp.9392-9401
- DOI
- 10.1523/JNEUROSCI.0124-10.2010
- PMID
- 20631168
- PMCID
- PMC3069688
- NLM abbreviation
- J Neurosci
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Publisher
- Society for Neuroscience
- Language
- English
- Date published
- 07/14/2010
- Academic Unit
- Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984070222802771
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