Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation

Xiaodong Tan; Israt Jahan; Yingyue Xu; Stuart Stock; Changyow Claire Kwan; Carmen Soriano; Xianghui Xiao; Jaime García-Añoveros; Bernd Fritzsch; Claus-Peter Richter

doi:10.1038/s41598-017-18814-9

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Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation

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Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation

Xiaodong Tan, Israt Jahan, Yingyue Xu, Stuart Stock, Changyow Claire Kwan, Carmen Soriano, Xianghui Xiao, Jaime García-Añoveros, Bernd Fritzsch and Claus-Peter Richter

Scientific reports, Vol.8(1), pp.388-388

01/10/2018

DOI: 10.1038/s41598-017-18814-9

PMCID: PMC5762820

PMID: 29321651

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Abstract

To determine whether responses during infrared neural stimulation (INS) result from the direct interaction with spiral ganglion neurons (SGNs), we tested three genetically modified deaf mouse models: Atoh1-cre; Atoh1 (Atoh1 conditional knockout, CKO), Atoh1-cre; Atoh1 (Neurog1 knockin, KI), and the Vglut3 knockout (Vglut3 ) mice. All animals were exposed to tone bursts and clicks up to 107 dB (re 20 µPa) and to INS, delivered with a 200 µm optical fiber. The wavelength (λ) was 1860 nm, the radiant energy (Q) 0-800 µJ/pulse, and the pulse width (PW) 100-500 µs. No auditory responses to acoustic stimuli could be evoked in any of these animals. INS could not evoke auditory brainstem responses in Atoh1 CKO mice but could in Neurog1 KI and Vglut3 mice. X-ray micro-computed tomography of the cochleae showed that responses correlated with the presence of SGNs and hair cells. Results in Neurog1 KI mice do not support a mechanical stimulation through the vibration of the basilar membrane, but cannot rule out the direct activation of the inner hair cells. Results in Vglut3 mice, which have no synaptic transmission between inner hair cells and SGNs, suggested that hair cells are not required.

Basic Helix-Loop-Helix Transcription Factors - genetics

Deafness - genetics

Acoustic Stimulation

Spiral Ganglion - physiology

Male

Gene Knockout Techniques

X-Ray Microtomography

Deafness - etiology

Deep Brain Stimulation - methods

Animals

Evoked Potentials, Auditory, Brain Stem

Hair Cells, Auditory - physiology

Infrared Rays

Amino Acid Transport Systems, Acidic - genetics

Mice

Deafness - therapy

Deafness - congenital

Disease Models, Animal

Details

Title: Subtitle: Auditory Neural Activity in Congenitally Deaf Mice Induced by Infrared Neural Stimulation
Creators: Xiaodong Tan - Department of Otolaryngology, Northwestern University Feinberg School of Medicine, 320 E. Chicago Avenue, Searle 12-561, Chicago, IL, 60611, USA
Israt Jahan - Department of Biology, University of Iowa, 129 E. Jefferson Street, Iowa City, IA, 52242, USA
Yingyue Xu - Department of Otolaryngology, Northwestern University Feinberg School of Medicine, 320 E. Chicago Avenue, Searle 12-561, Chicago, IL, 60611, USA
Stuart Stock - Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, 303 E. Chicago Avenue, Chicago, IL, 60611, USA
Changyow Claire Kwan - Department of Otolaryngology, Northwestern University Feinberg School of Medicine, 320 E. Chicago Avenue, Searle 12-561, Chicago, IL, 60611, USA
Carmen Soriano - Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL, 60439, USA
Xianghui Xiao - Advanced Photon Source, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL, 60439, USA
Jaime García-Añoveros - Departments of Anesthesiology, Physiology, and Neurology, Northwestern University Institute for Neuroscience, Ward 10-070, 303 East Chicago Avenue, Chicago, IL, 60611, USA
Bernd Fritzsch - Department of Biology, University of Iowa, 129 E. Jefferson Street, Iowa City, IA, 52242, USA
Claus-Peter Richter - The Hugh Knowles Center, Department of Communication Sciences and Disorders, Northwestern University, Frances Searle Building, 2240 Campus Drive, Evanston, IL, 60208, USA. cri529@northwestern.edu
Resource Type: Journal article
Publication Details: Scientific reports, Vol.8(1), pp.388-388
DOI: 10.1038/s41598-017-18814-9
PMID: 29321651
PMCID: PMC5762820
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Publisher: England
Grant note: R03 DC013655 / NIDCD NIH HHS R01 DC011855 / NIDCD NIH HHS
Language: English
Date published: 01/10/2018
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070310202771

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