Journal article
Augmentation of endogenous dopamine production increases lung liquid clearance
American journal of respiratory and critical care medicine, Vol.169(6), pp.757-763
2004
DOI: 10.1164/rccm.200207-744OC
PMID: 14701706
Abstract
We have previously reported that dopamine increased active Na+ transport in rat lungs by upregulating the alveolar epithelial Na,K-ATPase. Here we tested whether alveolar epithelial cells produce dopamine and whether increasing endogenous dopamine production by feeding rats a 4% tyrosine diet (TSD) would increase lung liquid clearance. Alveolar Type II cells express the enzyme aromatic-L-amino acid decarboxylase (AADC) and, when incubated with the dopamine precursor, 3-hydroxy-L-tyrosine (L-dopa), produce dopamine. Rats fed TSD, a precursor of L-dopa and dopamine, had increased urinary dopamine levels, which were inhibited by benserazide, an inhibitor of AADC. Rats fed TSD for 15, 24, and 48 hours had a 26, 46, and 45% increase in lung liquid clearance, respectively, as compared with controls. Also, dopaminergic D1 receptor antagonist--but not dopaminergic D2 receptor antagonist--inhibited the TSD-mediated increase in lung liquid clearance. Alveolar Type II cells isolated from the lungs of rats after they had been fed TSD for 24 hours demonstrated increased protein abundance of Na,K-ATPase alpha1 and beta1 subunits. Basolateral membranes isolated from peripheral lung tissue of tyrosine-fed rats had increased Na,K-ATPase activity and Na,K-ATPase alpha1 subunit. These data provide the first evidence that alveolar epithelial cells produce dopamine and that increasing endogenous dopamine increases lung liquid clearance.
Details
- Title: Subtitle
- Augmentation of endogenous dopamine production increases lung liquid clearance
- Creators
- Yochai ADIR - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenZaher S AZZAM - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenJacob Lasha SZNAJDER - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenEmilia LECUONA - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenSergio LEAL - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenLiuska PESCE - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenVidas DUMASIUS - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenAlejandro M BERTORELLO - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenPhillip FACTOR - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenJames B YOUNG - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, SwedenKaren M RIDGE - Department of Medicine, Atherosclerosis Research Unit, Karolinska Institutet, Karolinska Hospital, Stockholm, Sweden
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory and critical care medicine, Vol.169(6), pp.757-763
- Publisher
- American Lung Association
- DOI
- 10.1164/rccm.200207-744OC
- PMID
- 14701706
- ISSN
- 1073-449X
- eISSN
- 1535-4970
- Language
- English
- Date published
- 2004
- Academic Unit
- Endocrinology and Diabetes; Stead Family Department of Pediatrics
- Record Identifier
- 9984093325902771
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