Journal article
Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies
Journal of neuroimmunology, Vol.309, pp.41-46
08/15/2017
DOI: 10.1016/j.jneuroim.2017.05.002
PMID: 28601286
Abstract
Autoantibodies to nodal/paranodal proteins have been reported in patients with chronic inflammatory demyelinating polyneuropathy (CIDP) and multifocal motor neuropathy (MMN). To determine the frequency of anti-paranodal antibodies in our cohort of CIDP patients and to validate the presence anti-nodal antibodies in MMN, sera were screened for IgG against human neurofascin 155, contactin-1, neurofascin 186 and gliomedin using ELISA. In CIDP patients, 7% were anti-NF155 IgG4 positive and 7% were anti-CNTN1 IgG4 positive. Positive results were confirmed using cell based assays and indirect immunofluorescence on teased nerve fibres. We did not detect IgG autoantibodies against these nodal/paranodal antigens in MMN patients.
Detection of anti-NF155 and anti-CNTN1 antibodies. [Display omitted]
•Anti-NF155, anti-NF186, anti-gliomedin and anti-contactin IgG reactivity was measured by ELISA in CIDP and MMN patients•Positive IgG responses were confirmed using cell-based assays and teased nerve fibres•Standard ELISA using commercially available, recombinant human proteins is useful for the initial screening of patient sera
Details
- Title: Subtitle
- Autoantibody responses to nodal and paranodal antigens in chronic inflammatory neuropathies
- Creators
- E.K Mathey - Brain & Mind Centre, University of Sydney, Sydney, AustraliaN Garg - Brain & Mind Centre, University of Sydney, Sydney, AustraliaS.B Park - Brain & Mind Centre, University of Sydney, Sydney, AustraliaT Nguyen - Brain & Mind Centre, University of Sydney, Sydney, AustraliaS Baker - Department of Medicine, Physical Medicine & Neurology, Neuromuscular Disease Clinic, McMaster University, Hamilton, ON, CanadaN Yuki - Department of Neurology, Mishima Hospital, Niigata, JapanC Yiannikas - Northern Clinical School, University of Sydney, Sydney, AustraliaC.S Lin - School of Medical Sciences, Department of Physiology, Translational Neuroscience Facilities, University of New South Wales, New South Wales, AustraliaJ.M Spies - Institute of Clinical Neurosciences, Royal Prince Alfred Hospital, Sydney, AustraliaR Ghaoui - Neurology Department, Royal Adelaide Hospital, Adelaide, South Australia, AustraliaM.H Barnett - Brain & Mind Centre, University of Sydney, Sydney, AustraliaS Vucic - Western Clinical School, University of Sydney, New South Wales, AustraliaJ.D Pollard - Brain & Mind Centre, University of Sydney, Sydney, AustraliaM.C Kiernan - Brain & Mind Centre, University of Sydney, Sydney, Australia
- Resource Type
- Journal article
- Publication Details
- Journal of neuroimmunology, Vol.309, pp.41-46
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jneuroim.2017.05.002
- PMID
- 28601286
- ISSN
- 0165-5728
- eISSN
- 1872-8421
- Language
- English
- Date published
- 08/15/2017
- Academic Unit
- Iowa Neuroscience Institute; Biochemistry and Molecular Biology; Ophthalmology and Visual Sciences
- Record Identifier
- 9984071718202771
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