Autocrine Interferon Priming in Macrophages but Not Dendritic Cells Results in Enhanced Cytokine and Chemokine Production after Coronavirus Infection

Haixia Zhou; Jincun Zhao; Stanley Perlman

doi:10.1128/mBio.00219-10

Back

Autocrine Interferon Priming in Macrophages but Not Dendritic Cells Results in Enhanced Cytokine and Chemokine Production after Coronavirus Infection

Journal article

Open access

Autocrine Interferon Priming in Macrophages but Not Dendritic Cells Results in Enhanced Cytokine and Chemokine Production after Coronavirus Infection

Haixia Zhou, Jincun Zhao and Stanley Perlman

mBio, Vol.1(4), pp.e00219-e00219

10/19/2010

DOI: 10.1128/mBio.00219-10

PMCID: PMC2957079

PMID: 20978536

Files and links (1)

url

https://doi.org/10.1128/mBio.00219-10View

Published (Version of record) Open Access

Abstract

Coronaviruses efficiently inhibit interferon (IFN) induction in nonhematopoietic cells and conventional dendritic cells (cDC). However, IFN is produced in infected macrophages, microglia, and plasmacytoid dendritic cells (pDC). To begin to understand why IFN is produced in infected macrophages, we infected bone marrow-derived macrophages (BMM) and as a control, bone marrow-derived DC (BMDC) with the coronavirus mouse hepatitis virus (MHV). As expected, BMM but not BMDC expressed type I IFN. IFN production in infected BMM was nearly completely dependent on signaling through the alpha/beta interferon (IFN-α/β) receptor (IFNAR). Several IFN-dependent cytokines and chemokines showed the same expression pattern, with enhanced production in BMM compared to BMDC and dependence upon signaling through the IFNAR. Exogenous IFN enhanced IFN-dependent gene expression in BMM at early times after infection and in BMDC at all times after infection but did not stimulate expression of molecules that signal through myeloid differentiation factor 88 (MyD88), such as tumor necrosis factor (TNF). Collectively, our results show that IFN is produced at early times postinfection (p.i.) in MHV-infected BMM, but not in BMDC, and primes expression of IFN and IFN-responsive genes. Further, our results also show that BMM are generally more responsive to MHV infection, since MyD88-dependent pathways are also activated to a greater extent in these cells than in BMDC.

Cell Line

Dendritic Cells - immunology

Humans

Mice, Inbred C57BL

Cells, Cultured

Interferons - immunology

Coronavirus Infections - immunology

Murine hepatitis virus - physiology

Coronavirus - immunology

Mice, Knockout

Murine hepatitis virus - immunology

Animals

Macrophages - virology

Coronavirus Infections - virology

Mice

Dendritic Cells - virology

Coronavirus - physiology

Macrophages - immunology

Chemokines - immunology

Cytokines - immunology

Details

Title: Subtitle: Autocrine Interferon Priming in Macrophages but Not Dendritic Cells Results in Enhanced Cytokine and Chemokine Production after Coronavirus Infection
Creators: Haixia Zhou - Department of Microbiology, University of Iowa, Iowa City, Iowa, USA
Jincun Zhao
Stanley Perlman
Resource Type: Journal article
Publication Details: mBio, Vol.1(4), pp.e00219-e00219
DOI: 10.1128/mBio.00219-10
PMID: 20978536
PMCID: PMC2957079
NLM abbreviation: mBio
ISSN: 2150-7511
eISSN: 2150-7511
Publisher: United States
Grant note: NS36592 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 10/19/2010
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777472202771

Metrics

19 Record Views

36 Times Cited - Web of Science

See more details