Journal article
Autoimmunity in HLA-DQ8 transgenic mice expressing granulocyte/macrophage-colony stimulating factor in the beta cells of islets of langerhans
Autoimmunity, Vol.40(3), pp.169-179
01/01/2007
DOI: 10.1080/08916930701201083
PMID: 17453715
Abstract
Type 1 diabetes (T1D) is a polygenic autoimmune disease with a strong HLA association particularly, HLA-DQ8. We investigated whether islet-specific expression of granulocyte/macrophage colony-stimulating factor (Ins.GM-CSF) in Aß°.NOD.DQ8 mice (HLA-DQ8 transgenic mice on a NOD background lacking endogenous mouse MHC class II molecules) would predispose to development of spontaneous autoimmune diabetes. Aß°.NOD.DQ8 mice expressing GM-CSF in the pancreatic ß cells (8+ G+) as well as litter mates lacking either HLA-DQ8 (8 − G+) or GM-CSF (8+ G − ) or both (8 − G − ) exhibited insulitis and sialadenitis of varying degrees. But none of the mice progressed to develop T1D. Other than the marked mononuclear cell infiltration in livers of mice expressing GM-CSF irrespective of HLA-DQ8 expression (8+ G+ or 8 − G+), no other changes were observed in the animals. Thus, we have shown for the first time that expression of HLA-DQ8 in the diabetes-predisposing mileu of NOD genetic background is not sufficient to predispose to development of autoimmune diabetes even when the potent immunostimulatory cytokine, GM-CSF is expressed in the pancreatic islets.
Details
- Title: Subtitle
- Autoimmunity in HLA-DQ8 transgenic mice expressing granulocyte/macrophage-colony stimulating factor in the beta cells of islets of langerhans
- Creators
- Govindarajan RajagopalanAshutosh K MangalamMoon M SenShen ChengYogish C KudvaChella S David
- Resource Type
- Journal article
- Publication Details
- Autoimmunity, Vol.40(3), pp.169-179
- DOI
- 10.1080/08916930701201083
- PMID
- 17453715
- NLM abbreviation
- Autoimmunity
- ISSN
- 0891-6934
- eISSN
- 1607-842X
- Publisher
- Taylor & Francis
- Language
- English
- Date published
- 01/01/2007
- Academic Unit
- Pathology; Iowa Neuroscience Institute
- Record Identifier
- 9983905530502771
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