Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates

John A Widness; Denison J Kuruvilla; Donald M Mock; Nell I Matthews; Demet Nalbant; Gretchen A Cress; Robert L Schmidt; Ronald G Strauss; M Bridget Zimmerman; Peter Veng-Pedersen

doi:10.1016/j.jpeds.2015.08.028

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Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates

Journal article

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Peer reviewed

Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates

John A Widness, Denison J Kuruvilla, Donald M Mock, Nell I Matthews, Demet Nalbant, Gretchen A Cress, Robert L Schmidt, Ronald G Strauss, M Bridget Zimmerman and Peter Veng-Pedersen

The Journal of pediatrics, Vol.167(5), pp.1001-1006

11/2015

DOI: 10.1016/j.jpeds.2015.08.028

PMCID: PMC4661104

PMID: 26363547

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Abstract

Based on the hypothesis that neonatal autologous red blood cell (RBC) survival (RCS) is substantially shorter than adult RBC, we concurrently tracked the survival of transfused biotin-labeled autologous neonatal and allogeneic adult RBC into ventilated, very low birth weight infants. RBC aliquots from the first clinically ordered, allogeneic adult RBC transfusion and from autologous infant blood were labeled at separate biotin densities (biotin-labeled RBC [BioRBC]) and transfused. Survival of these BioRBCs populations were concurrently followed over weeks by flow cytometric enumeration using leftover blood. Relative tracking of infant autologous and adult allogeneic BioRBC was analyzed by linear mixed modeling of batched weekly data. When possible, Kidd antigen (Jka and Jkb) mismatches between infant and donor RBCs were also used to track these 2 populations. Contrary to our hypothesis, concurrent tracking curves of RCS of neonatal and adult BioRBC in 15 study infants did not differ until week 7, after which neonatal RCS became shortened to 59%-79% of adult enumeration values for uncertain reasons. Analysis of mismatched Kidd antigen RBC showed similar results, thus, confirming that BioRBC tracking is not perturbed by biotin RBC labeling. This study illustrates the utility of multidensity BioRBC labeling for concurrent measurement of RCS of multiple RBC populations in vivo. The similar RCS results observed for neonatal and adult BioRBCs transfused into very low birth weight infants provides strong evidence that the circulatory environment of the newborn infant, not intrinsic infant-adult RBC differences, is the primary determinant of erythrocyte survival. Clinicaltrials.gov: NCT00731588.

Erythropoiesis

Models, Theoretical

Biotinylation

Prospective Studies

Cell Survival

Infant, Very Low Birth Weight

Kidd Blood-Group System

Humans

Male

Transplantation, Autologous

Erythrocyte Transfusion - methods

Transplantation, Homologous

Biotin - chemistry

Flow Cytometry

Infant, Premature

Adult

Female

Infant, Newborn

Details

Title: Subtitle: Autologous Infant and Allogeneic Adult Red Cells Demonstrate Similar Concurrent Post-Transfusion Survival in Very Low Birth Weight Neonates
Creators: John A Widness - Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA. Electronic address: john-widness@uiowa.edu
Denison J Kuruvilla - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA
Donald M Mock - Department of Biochemistry and Molecular Biology and the Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR
Nell I Matthews - Department of Biochemistry and Molecular Biology and the Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR
Demet Nalbant - Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA
Gretchen A Cress - Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA
Robert L Schmidt - Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA
Ronald G Strauss - Department of Pediatrics, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA; Department of Pathology, The University of Iowa, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA
M Bridget Zimmerman - Department of Biostatistics, University of Iowa College of Public Health, Iowa City, IA
Peter Veng-Pedersen - Department of Pharmaceutical Sciences and Experimental Therapeutics, University of Iowa College of Pharmacy, Iowa City, IA
Resource Type: Journal article
Publication Details: The Journal of pediatrics, Vol.167(5), pp.1001-1006
DOI: 10.1016/j.jpeds.2015.08.028
PMID: 26363547
PMCID: PMC4661104
NLM abbreviation: J Pediatr
ISSN: 0022-3476
eISSN: 1097-6833
Publisher: United States
Grant note: UL1 TR000039 / NCATS NIH HHS S10 RR027219 / NCRR NIH HHS UL1RR024979 / NCRR NIH HHS 1S10RR027219 / NCRR NIH HHS P01 HL046925 / NHLBI NIH HHS UL1 RR024979 / NCRR NIH HHS UL1TR000039 / NCATS NIH HHS
Language: English
Date published: 11/2015
Academic Unit: Stead Family Department of Pediatrics; Pathology; Pharmaceutical Sciences and Experimental Therapeutics; Biostatistics; Gastroenterology, Hepatology, Pancreatology, and Nutrition
Record Identifier: 9983997370302771

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