Journal article
Autologous NeoHep Derived from Chronic Hepatitis B Virus Patients’ Blood Monocytes by Upregulation of c‐MET Signaling
Stem cells translational medicine, Vol.6(1), pp.174-186
01/2017
DOI: 10.5966/sctm.2015-0308
PMID: 28170202
Abstract
In view of the escalating need for autologous cell‐based therapy for treatment of liver diseases, a novel candidate has been explored in the present study. The monocytes isolated from hepatitis B surface antigen (HBsAg) nucleic acid test (NAT)‐positive (HNP) blood were differentiated to hepatocyte‐like cells (NeoHep) in vitro by a two‐step culture procedure. The excess neutrophils present in HNP blood were removed before setting up the culture. In the first step of culture, apoptotic cells were depleted and genes involved in hypoxia were induced, which was followed by the upregulation of genes involved in the c‐MET signaling pathway in the second step. The NeoHep were void of hepatitis B virus and showed expression of albumin, connexin 32, hepatocyte nuclear factor 4‐α, and functions such as albumin secretion and cytochrome P450 enzyme‐mediated detoxification of xenobiotics. The engraftment of NeoHep derived from HBsAg‐NAT‐positive blood monocytes in partially hepatectomized NOD.CB17‐Prkdcscid/J mice liver and the subsequent secretion of human albumin and clotting factor VII activity in serum make NeoHep a promising candidate for cell‐based therapy. Stem Cells Translational Medicine 2017;6:174–186
Details
- Title: Subtitle
- Autologous NeoHep Derived from Chronic Hepatitis B Virus Patients’ Blood Monocytes by Upregulation of c‐MET Signaling
- Creators
- Jashdeep Bhattacharjee - National Institute of ImmunologyBarun Das - National Institute of ImmunologyDisha Sharma - Institute of Genomics and Integrative BiologyPreeti Sahay - National Institute of ImmunologyKshama Jain - National Institute of ImmunologyAlaknanda Mishra - National Institute of ImmunologySrikanth Iyer - National Institute of ImmunologyPuja Nagpal - National Institute of ImmunologyVinod Scaria - Institute of Genomics and Integrative BiologyPerumal Nagarajan - National Institute of ImmunologyPrakash Khanduri - St. Stephen’s HospitalAsok Mukhopadhyay - National Institute of ImmunologyPramod Upadhyay - National Institute of Immunology
- Resource Type
- Journal article
- Publication Details
- Stem cells translational medicine, Vol.6(1), pp.174-186
- DOI
- 10.5966/sctm.2015-0308
- PMID
- 28170202
- NLM abbreviation
- Stem Cells Transl Med
- ISSN
- 2157-6564
- eISSN
- 2157-6580
- Publisher
- John Wiley and Sons Inc
- Number of pages
- 13
- Alternative title
- Autologous NeoHep from Chronic HBV Monocytes
- Language
- English
- Date published
- 01/2017
- Academic Unit
- Surgery
- Record Identifier
- 9985244683002771
Metrics
1 Record Views