Automated detection of squint as a sensitive assay of sex-dependent CGRP and amylin-induced pain in mice

Brandon J Rea; Abigail Davison; Martin-Junior Ketcha; Kylie J Smith; Aaron M Fairbanks; Anne-Sophie Wattiez; Pieter Poolman; Randy H Kardon; Andrew F Russo; Levi P Sowers

doi:10.1097/j.pain.0000000000002537

Back

Automated detection of squint as a sensitive assay of sex-dependent CGRP and amylin-induced pain in mice

Journal article

Peer reviewed

Automated detection of squint as a sensitive assay of sex-dependent CGRP and amylin-induced pain in mice

Brandon J Rea, Abigail Davison, Martin-Junior Ketcha, Kylie J Smith, Aaron M Fairbanks, Anne-Sophie Wattiez, Pieter Poolman, Randy H Kardon, Andrew F Russo and Levi P Sowers

Pain (Amsterdam), Vol.163(8), pp.1511-1519

08/2022

DOI: 10.1097/j.pain.0000000000002537

PMCID: PMC9085964

PMID: 34772897

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/9085964View

Open Access

Abstract

We developed an automated squint assay using both black C57BL/6J and white CD1 mice to measure the interpalpebral fissure area between the upper and lower eyelids as an objective quantification of pain. The automated software detected a squint response to the commonly used nociceptive stimulus formalin in C57BL/6J mice. After this validation, we used the automated assay to detect a dose-dependent squint response to a migraine trigger, the neuropeptide calcitonin gene–related peptide, including a response in female mice at a dose below detection by the manual grimace scale. Finally, we found that the calcitonin gene–related peptide amylin induced squinting behavior in female mice, but not males. These data demonstrate that an automated squint assay can be used as an objective, real-time, continuous-scale measure of pain that provides higher precision and real-time analysis compared with manual grimace assessments.

Details

Title: Subtitle: Automated detection of squint as a sensitive assay of sex-dependent CGRP and amylin-induced pain in mice
Creators: Brandon J Rea
Abigail Davison
Martin-Junior Ketcha
Kylie J Smith
Aaron M Fairbanks
Anne-Sophie Wattiez
Pieter Poolman
Randy H Kardon
Andrew F Russo
Levi P Sowers
Resource Type: Journal article
Publication Details: Pain (Amsterdam), Vol.163(8), pp.1511-1519
DOI: 10.1097/j.pain.0000000000002537
PMID: 34772897
PMCID: PMC9085964
NLM abbreviation: Pain
ISSN: 0304-3959
eISSN: 1872-6623
Language: English
Electronic publication date: 11/10/2021
Date published: 08/2022
Academic Unit: Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center; Neurology (Pediatrics); Ophthalmology and Visual Sciences
Record Identifier: 9984197395502771

Metrics

29 Record Views

22 Times Cited - Web of Science