Journal article
Autophagy Quantification and STAT3 Expression in a Human Skin Organ Culture Model for Innate Immunity to Herpes Zoster
Frontiers in microbiology, Vol.9, pp.2935-2935
12/05/2018
DOI: 10.3389/fmicb.2018.02935
PMCID: PMC6290052
PMID: 30568636
Abstract
The goal of this project was to document the autophagy response in human neonatal skin organ culture (SOC) after infection with varicella-zoster virus (VZV). The VZV-infected SOC model has attributes of herpes zoster, in that an injection of virus into the skin is analogous to exit of virus from the sensory nerve termini into skin during herpes zoster. Cultures were maintained for 28 days and periodically examined for an autophagy response by quantitation of autophagosomes with Imaris software. Expression of the STAT3 protein was plentiful in the VZV-infected SOC. Abundant autophagy was observed in VZV-infected SOC between 14 and 28 days after infection, while autophagy in mock-infected SOC was minimal (
p
= 0.0003). The autophagic response after infection of SOC with a recombinant VZV genome containing the herpes simplex virus ICP34.5 neurovirulence gene was similar to wild-type VZV (
p
= 0.3). These results suggested that the VZV-infected SOC system resembled biopsy data from herpes zoster infection of skin. An enhanced autophagy response has now been reported after infection with two additional alpha herpesviruses besides VZV, namely, pseudorabies virus and duck enteritis herpes virus; both lack the ICP34.5 protein.
Details
- Title: Subtitle
- Autophagy Quantification and STAT3 Expression in a Human Skin Organ Culture Model for Innate Immunity to Herpes Zoster
- Creators
- Erin M Buckingham - Virology Laboratory, Children’s Hospital, University of IowaJames Girsch - Virology Laboratory, Children’s Hospital, University of IowaWallen Jackson - Virology Laboratory, Children’s Hospital, University of IowaJeffrey I Cohen - Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of HealthCharles Grose - Virology Laboratory, Children’s Hospital, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Frontiers in microbiology, Vol.9, pp.2935-2935
- DOI
- 10.3389/fmicb.2018.02935
- PMID
- 30568636
- PMCID
- PMC6290052
- NLM abbreviation
- Front Microbiol
- ISSN
- 1664-302X
- eISSN
- 1664-302X
- Publisher
- Frontiers Media S.A
- Grant note
- AI89716; HD94333 / National Institutes of Health
- Language
- English
- Date published
- 12/05/2018
- Academic Unit
- Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
- Record Identifier
- 9984093355602771
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