Journal article
Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages
The Journal of cell biology, Vol.186(2), pp.255-268
07/27/2009
DOI: 10.1083/jcb.200903070
PMCID: PMC2717652
PMID: 19635843
Abstract
Autophagy is a cytoplasmic degradative pathway that can participate in biosynthetic processes, as in the yeast Cvt pathway, but is more commonly known for its functions in removing damaged or surplus organelles and macromolecular complexes. Here, we find that autophagy intersects with human immunodeficiency virus (HIV) biogenesis, mirroring the above dichotomy. Early, nondegradative stages of autophagy promoted HIV yields. HIV Gag-derived proteins colocalized and interacted with the autophagy factor LC3, and autophagy promoted productive Gag processing. Nevertheless, when autophagy progressed through maturation stages, HIV was degraded. This, however, does not occur, as the HIV protein Nef acts as an antiautophagic maturation factor through interactions with the autophagy regulatory factor Beclin 1, thus protecting HIV from degradation. The dual interaction of HIV with the autophagy pathway enhances viral yields by using the early stages while inhibiting the late stages of autophagy. The role of Nef in the latter process enhances yields of infectious HIV and may be of significance for progression to clinical AIDS.
Details
- Title: Subtitle
- Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages
- Creators
- George B Kyei - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Christina Dinkins - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Alexander S Davis - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Esteban Roberts - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Sudha B Singh - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Chunsheng Dong - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226Li Wu - Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226Eiki Kominami - Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113-8421, JapanTakashi Ueno - Department of Biochemistry, Juntendo University School of Medicine, Tokyo 113-8421, JapanAkitsugu Yamamoto - Nagahama Institute of Bio-Science and Technology, Tamura 1266, Nagahama, Shiga 526-0829, JapanMaurizio Federico - National AIDS Center, Istituto Superiore di Sanita, 00161 Rome, ItalyAntonito Panganiban - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Isabelle Vergne - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131Vojo Deretic - Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131, Department of Molecular Genetics and Microbiology and Department of Cell Biology and Physiology, University of New Mexico School of Medicine, Albuquerque, NM 87131
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.186(2), pp.255-268
- DOI
- 10.1083/jcb.200903070
- PMID
- 19635843
- PMCID
- PMC2717652
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 07/27/2009
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984001126002771
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