Journal article
Avian lungs: A novel scaffold for lung bioengineering
PloS one, Vol.13(6), pp.e0198956-e0198956
2018
DOI: 10.1371/journal.pone.0198956
PMCID: PMC6021073
PMID: 29949597
Abstract
Allogeneic lung transplant is limited both by the shortage of available donor lungs and by the lack of suitable long-term lung assist devices to bridge patients to lung transplantation. Avian lungs have different structure and mechanics resulting in more efficient gas exchange than mammalian lungs. Decellularized avian lungs, recellularized with human lung cells, could therefore provide a powerful novel gas exchange unit for potential use in pulmonary therapeutics. To initially assess this in both small and large avian lung models, chicken (Gallus gallus domesticus) and emu (Dromaius novaehollandiae) lungs were decellularized using modifications of a detergent-based protocol, previously utilized with mammalian lungs. Light and electron microscopy, vascular and airway resistance, quantitation and gel analyses of residual DNA, and immunohistochemical and mass spectrometric analyses of remaining extracellular matrix (ECM) proteins demonstrated maintenance of lung structure, minimal residual DNA, and retention of major ECM proteins in the decellularized scaffolds. Seeding with human bronchial epithelial cells, human pulmonary vascular endothelial cells, human mesenchymal stromal cells, and human lung fibroblasts demonstrated initial cell attachment on decellularized avian lungs and growth over a 7-day period. These initial studies demonstrate that decellularized avian lungs may be a feasible approach for generating functional lung tissue for clinical therapeutics.
Details
- Title: Subtitle
- Avian lungs: A novel scaffold for lung bioengineering
- Creators
- Sean M Wrenn - Department of Medicine, University of Vermont, Burlington, VT, United States of AmericaEthan D Griswold - Rochester Institute of Technology, Rochester, NY, United States of AmericaFranziska E Uhl - Department of Medicine, University of Vermont, Burlington, VT, United States of AmericaJuan J Uriarte - Department of Medicine, University of Vermont, Burlington, VT, United States of AmericaHeon E Park - Department of Mechanical Engineering, University of Vermont, Burlington, VT, United States of AmericaAmy L Coffey - Department of Medicine, University of Vermont, Burlington, VT, United States of AmericaJacob S Dearborn - Department of Medicine, University of Vermont, Burlington, VT, United States of AmericaBethany A Ahlers - University of Iowa, Anatomy and Cell BiologyBin Deng - Department of Biology, University of Vermont, Burlington, VT, United States of AmericaYing-Wai Lam - Department of Biology, University of Vermont, Burlington, VT, United States of AmericaDryver R Huston - Department of Mechanical Engineering, University of Vermont, Burlington, VT, United States of AmericaPatrick C Lee - Department of Mechanical Engineering, University of Vermont, Burlington, VT, United States of AmericaDarcy E Wagner - Department of Experimental Medical Science, Lung Bioengineering and Regeneration, Lund University, Lund, SwedenDaniel J Weiss - University of North Carolina at Chapel Hill
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.13(6), pp.e0198956-e0198956
- DOI
- 10.1371/journal.pone.0198956
- PMID
- 29949597
- PMCID
- PMC6021073
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Grant note
- R21 EB024329 / NIBIB NIH HHS
- Language
- English
- Date published
- 2018
- Academic Unit
- Anatomy and Cell Biology; Cardiothoracic Surgery
- Record Identifier
- 9984131899202771
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