Axonal damage is T cell mediated and occurs concomitantly with demyelination in mice infected with a neurotropic coronavirus

A A Dandekar; G F Wu; L Pewe; S Perlman

doi:10.1128/JVI.75.13.6115-6120.2001

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Axonal damage is T cell mediated and occurs concomitantly with demyelination in mice infected with a neurotropic coronavirus

Journal article

Open access

Peer reviewed

Axonal damage is T cell mediated and occurs concomitantly with demyelination in mice infected with a neurotropic coronavirus

A A Dandekar, G F Wu, L Pewe and S Perlman

Journal of virology, Vol.75(13), pp.6115-6120

07/2001

DOI: 10.1128/JVI.75.13.6115-6120.2001

PMCID: PMC114327

PMID: 11390613

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https://europepmc.org/articles/pmc114327View

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Abstract

Mice infected with mouse hepatitis virus (MHV) strain JHM develop primary demyelination. Herein we show that axonal damage occurred in areas of demyelination and also in adjacent areas devoid of myelin damage. Immunodeficient MHV-infected RAG1-/- mice (mice defective in recombinase activating gene 1 expression) do not develop demyelination unless they receive splenocytes from a mouse previously immunized against MHV (G. F. Wu, A. Dandekar, L. Pewe, and S. Perlman, J. Immunol. 165:2278-2286, 2000). In the present study, we show that adoptive transfer of T cells was also required for the majority of the axonal injury observed in these animals. Both demyelination and axonal damage were apparent by 7 days posttransfer. Recent data suggest that axonal injury is a major factor in the long-term disability observed in patients with multiple sclerosis. Our data demonstrate that immune system-mediated damage to axons is also a common feature in mice with MHV-induced demyelination. Remarkably, there appeared to be a minimal, if any, interval of time between the appearance of demyelination and that of axonal injury.

Immunohistochemistry

T-Lymphocytes - physiology

Animals

Spinal Cord - pathology

Axons - pathology

Neurofilament Proteins - analysis

Coronavirus Infections - pathology

Mice

Adoptive Transfer

Demyelinating Diseases - pathology

Homeodomain Proteins - physiology

Murine hepatitis virus

Details

Title: Subtitle: Axonal damage is T cell mediated and occurs concomitantly with demyelination in mice infected with a neurotropic coronavirus
Creators: A A Dandekar - Interdisciplinary Programs in Immunology, University of Iowa, Iowa City, IA 52242, USA
G F Wu
L Pewe
S Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.75(13), pp.6115-6120
DOI: 10.1128/JVI.75.13.6115-6120.2001
PMID: 11390613
PMCID: PMC114327
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: NS 36592 / NINDS NIH HHS NS 40438 / NINDS NIH HHS MH2066-02 / NIMH NIH HHS T32 GM007337 / NIGMS NIH HHS R01 NS040438 / NINDS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 07/2001
Academic Unit: Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777350402771

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