Journal article
Axons modulate the expression of transforming growth factor‐betas in Schwann cells
Glia, Vol.8(4), pp.265-276
1993
DOI: 10.1002/glia.440080407
PMID: 8406683
Abstract
We have investigated the expression of transforming growth factor (TGF)‐β1,‐β2, and ‐β3 in developing, degenerating, and regenerating rat peripheral nerve by immunohistochemistry and Northern blot analysis. In normal adult sciatic nerve, TGF‐β1, ‐β2, and ‐β3 are detected in the cytoplasm of Schwann cells, and the levels of TGF‐β1 and ‐β3 mRNAs are constant during post‐natal development. When sciatic nerves are transected to cause axonal degeneration and prevent axonal regeneration, the level of TGF‐β1 mRNA in the distal nerve‐stump increases markedly and remains elevated, whereas the level of TGF‐β3 mRNA falls modestly and remains depressed. When sciatic nerves are crushed to cause axonal degeneration and allow axonal regeneration, the level of TGF‐β1 mRNA initially increases as axons degenerate, and then falls as axons regenerate. TGF‐β2 mRNA was not detected in developing or lesioned sciatic nerves at any time. Cultured Schwann cells have high levels of TGF‐β1 mRNA, the amount of which is reduced by forskolin, which mimicks the effect of axonal contact. These data demonstrate that Schwann cells express TGF‐β1, ‐β2, and ‐β3, and that TGF‐β1 and ‐β3 mRNA predominate over TGF‐β2 mRNA in peripheral nerve. Axonal contact and forskolin decrease the expression of TGF‐β1 in Schwann cells. © 1993 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Axons modulate the expression of transforming growth factor‐betas in Schwann cells
- Creators
- Steven S SchererJohn KamholzSonia B Jakowlew
- Resource Type
- Journal article
- Publication Details
- Glia, Vol.8(4), pp.265-276
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/glia.440080407
- PMID
- 8406683
- ISSN
- 0894-1491
- eISSN
- 1098-1136
- Number of pages
- 12
- Language
- English
- Date published
- 1993
- Academic Unit
- Neurology; Psychiatry
- Record Identifier
- 9984020884302771
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