Journal article
B-ZIP Proteins Encoded by the Drosophila Genome: Evaluation of Potential Dimerization Partners
Genome research, Vol.12(8), pp.1190-1200
08/2002
DOI: 10.1101/gr.67902
PMCID: PMC186634
PMID: 12176927
Abstract
The basic region-leucine zipper (B-ZIP) (bZIP) protein motif dimerizes to bind specific DNA sequences. We have identified 27 B-ZIP proteins in the recently sequenced
Drosophila melanogaster
genome. The dimerization specificity of these 27 B-ZIP proteins was evaluated using two structural criteria: (1) the presence of attractive or repulsive interhelical g↔e‘ electrostatic interactions and (2) the presence of polar or charged amino acids in the ‘a’ and ‘d’ positions of the hydrophobic interface. None of the B-ZIP proteins contain only aliphatic amino acids in the‘a’ and ‘d’ position. Only six of the
Drosophila
B-ZIP proteins contain a “canonical” hydrophobic interface like the yeast GCN4, and the mammalian JUN, ATF2, CREB, C/EBP, and PAR leucine zippers, characterized by asparagine in the second ‘a’ position. Twelve leucine zippers contain polar amino acids in the first, third, and fourth ‘a’ positions. Circular dichroism spectroscopy, used to monitor thermal denaturations of a heterodimerizing leucine zipper system containing either valine (V) or asparagine (N) in the ‘a’ position, indicates that the V–N interaction is 2.3 kcal/mole less stable than an N–N interaction and 5.3 kcal/mole less stable than a V–V interaction. Thus, we propose that the presence of polar amino acids in novel positions of the ‘a’ position of
Drosophila
B-ZIP proteins has led to leucine zippers that homodimerize rather than heterodimerize.
Details
- Title: Subtitle
- B-ZIP Proteins Encoded by the Drosophila Genome: Evaluation of Potential Dimerization Partners
- Creators
- Jan Fassler - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USADavid Landsman - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USAAsha Acharya - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USAJonathan R Moll - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USAMaria Bonovich - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USACharles Vinson - Computational Biology Branch, National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Maryland 20814, USA; Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA; Department of Biological Sciences, University of Iowa, Iowa City, Iowa 52242, USA
- Resource Type
- Journal article
- Publication Details
- Genome research, Vol.12(8), pp.1190-1200
- Publisher
- Cold Spring Harbor Laboratory Press
- DOI
- 10.1101/gr.67902
- PMID
- 12176927
- PMCID
- PMC186634
- ISSN
- 1088-9051
- eISSN
- 1549-5469
- Language
- English
- Date published
- 08/2002
- Academic Unit
- Biology
- Record Identifier
- 9984217428202771
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