B cells express Ly-6C in a Th1 but not Th2 cytokine environment

Annette J Schlueter; Arthur M Krieg; Peter De Vries; Xiang Li

doi:10.1089/107999002320271396

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B cells express Ly-6C in a Th1 but not Th2 cytokine environment

Journal article

Peer reviewed

B cells express Ly-6C in a Th1 but not Th2 cytokine environment

Annette J Schlueter, Arthur M Krieg, Peter De Vries and Xiang Li

Journal of interferon & cytokine research, Vol.22(7), pp.799-806

07/2002

DOI: 10.1089/107999002320271396

PMID: 12184918

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Abstract

Interferon-alpha (IFN-alpha) is the primary regulator of transient Ly-6C expression on T cells. B cells, which do not express Ly-6C in the resting state, have been reported to express Ly-6C following exposure to proinflammatory stimuli. This study examined the factors controlling Ly-6C expression on B cells and the kinetics of Ly-6C expression in the presence of these factors. In vivo studies demonstrated that proinflammatory (Th1) cytokines transiently upregulate B cell Ly-6C expression. In vitro studies identified Th1 cytokines, particularly IFN-alpha and IFN-gamma, as the principal cytokines responsible for this induction. Polyclonal B cell activators (anti-IgM and recombinant CD40 ligand trimer) showed minimal ability to independently induce Ly-6C expression on B cells but did enhance the ability of IFNs to induce expression. Th2 cytokine environments did not result in B cell Ly-6C expression, and interleukin-4 (IL-4) actually antagonized the IFN-driven induction of Ly-6C. Ly6.1 strains of mice consistently demonstrated a greater ability to express Ly-6C on B cells than did Ly-6.2 strains. Together, these studies demonstrate the ability of Th1 but not Th2 cytokine environments to transiently induce the expression of Ly-6C on B cells and provide additional evidence for differences in the regulation of Ly-6C expression in Ly6.1 and Ly6.2 strains.

Immunization

CpG Islands - immunology

Antibodies, Anti-Idiotypic - pharmacology

Interferon-alpha - antagonists & inhibitors

Lymphokines - physiology

Mice, Inbred C57BL

Antigens, Ly - genetics

Gene Expression Regulation

Interferon-gamma - physiology

Spleen - cytology

Th2 Cells - immunology

Recombinant Proteins - pharmacology

Th1 Cells - immunology

Interleukin-4 - pharmacology

Animals

Antigens, Ly - biosynthesis

CD40 Ligand - pharmacology

Interferon-gamma - antagonists & inhibitors

Mice, Inbred DBA

Mice

Mice, Inbred BALB C

B-Lymphocytes - metabolism

Interferon-alpha - physiology

Details

Title: Subtitle: B cells express Ly-6C in a Th1 but not Th2 cytokine environment
Creators: Annette J Schlueter - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242-1181, USA. schluetera@uihc.uiowa.edu
Arthur M Krieg
Peter De Vries
Xiang Li
Resource Type: Journal article
Publication Details: Journal of interferon & cytokine research, Vol.22(7), pp.799-806
Publisher: United States
DOI: 10.1089/107999002320271396
PMID: 12184918
ISSN: 1079-9907
eISSN: 1557-7465
Language: English
Date published: 07/2002
Academic Unit: Pathology
Record Identifier: 9984047997502771

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