BAG4/SODD protein contains a short BAG domain

Klara Briknarová; Shinichi Takayama; Sachiko Homma; Kelly Baker; Edelmira Cabezas; David W Hoyt; Zhen Li; Arnold C Satterthwait; Kathryn R Ely

doi:10.1074/jbc.M202792200

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BAG4/SODD protein contains a short BAG domain

Journal article

Open access

Peer reviewed

BAG4/SODD protein contains a short BAG domain

Klara Briknarová, Shinichi Takayama, Sachiko Homma, Kelly Baker, Edelmira Cabezas, David W Hoyt, Zhen Li, Arnold C Satterthwait and Kathryn R Ely

The Journal of biological chemistry, Vol.277(34), pp.31172-31178

08/23/2002

DOI: 10.1074/jbc.M202792200

PMID: 12058034

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Abstract

BAG (Bcl-2-associated athanogene) proteins are molecular chaperone regulators that affect diverse cellular pathways. All members share a conserved motif, called the BAG domain (BD), which binds to Hsp70/Hsc70 family proteins and modulates their activity. We have determined the solution structure of BD from BAG4/SODD (silencer of death domains) by multidimensional nuclear magnetic resonance methods and compared it to the corresponding domain in BAG1 (Briknarová, K., Takayama, S., Brive, L., Havert, M. L., Knee, D. A., Velasco, J., Homma, S., Cabezas, E., Stuart, J., Hoyt, D. W., Satterthwait, A. C., Llinás, M., Reed, J. C., and Ely, K. R. (2001) Nat. Struct. Biol. 8, 349-352). The difference between BDs from these two BAG proteins is striking, and the structural comparison defines two subfamilies of mammalian BD-containing proteins. One subfamily includes the closely related BAG3, BAG4, and BAG5 proteins, and the other is represented by BAG1, which contains a structurally and evolutionarily distinct BD. BDs from both BAG1 and BAG4 are three-helix bundles; however, in BAG4, each helix in this bundle is three to four turns shorter than its counterpart in BAG1, which reduces the length of the domain by one-third. BAG4 BD thus represents a prototype of the minimal functional fragment that is capable of binding to Hsc70 and modulating its chaperone activity.

Adaptor Proteins, Signal Transducing

Amino Acid Sequence

Apoptosis Regulatory Proteins

Carrier Proteins - chemistry

Carrier Proteins - physiology

Conserved Sequence

DNA-Binding Proteins

HSC70 Heat-Shock Proteins

HSP70 Heat-Shock Proteins - metabolism

Humans

Membrane Proteins

Molecular Sequence Data

Sequence Alignment

Structure-Activity Relationship

Transcription Factors - chemistry

Details

Title: Subtitle: BAG4/SODD protein contains a short BAG domain
Creators: Klara Briknarová - Burnham Institute, La Jolla, CA 92037, USA
Shinichi Takayama
Sachiko Homma
Kelly Baker
Edelmira Cabezas
David W Hoyt
Zhen Li
Arnold C Satterthwait
Kathryn R Ely
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.277(34), pp.31172-31178
DOI: 10.1074/jbc.M202792200
PMID: 12058034
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Grant note: CA 67329 / NCI NIH HHS
Language: English
Date published: 08/23/2002
Academic Unit: Occupational and Environmental Health; Epidemiology
Record Identifier: 9984214947402771

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