Journal article
BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation
Journal of materials chemistry. B, Materials for biology and medicine, Vol.5(26), pp.5196-5205
2017
DOI: 10.1039/C7TB00744B
PMCID: PMC5730084
PMID: 29250330
Abstract
Bone morphogenetic proteins (BMPs,
e.g.
, BMP2 and 7) are potent mediators for bone repair, however, their clinical use has been limited by their safety and cost-effectiveness. Therefore, innovative strategies that can improve the efficacy of BMPs, and thereby use a lower dose of exogenous BMPs are highly desired. Inspired by the natural interactions between the extracellular matrix (ECM) and growth factors, we hypothesize that bone matrix-mimicking nanofibrous scaffolds functionalized with BMP binding moieties can selectively capture and stabilize BMPs, and thereby promote BMP-induced osteogenic differentiation. To test our hypothesis, a gelatin nanofibrous scaffold was fabricated using thermally induced phase separation together with a porogen leaching technique (TIPS&P) and functionalized by a BMP-binding peptide (BBP) through cross-linking. Our data indicated that BBP decoration largely improved the BMP2 binding and retention capacity of the nanofibrous scaffolds without compromising their macro/microstructure and mechanical properties. Importantly, the BBP-functionalized gelatin scaffolds were able to significantly promote BMP2-induced osteogenic differentiation. Moreover, the BBP alone was able to significantly stimulate endogenous BMP2 expression and improve osteogenic differentiation. Compared to other affinity-based drug delivery strategies,
e.g.
, heparin and antibody-mediated growth factor delivering techniques, we expect that BBP-functionalized scaffolds will be a safer, more feasible and selective strategy for endogenous BMP stimulating and binding. Therefore, our data suggest a promising application of using the BBP-decorated gelatin nanofibrous scaffolds to stimulate/capture BMPs and promote endogenous bone formation
in situ
in contrast to relying on the administration of high doses of exogenous BMPs and transplantation of cells.
Details
- Title: Subtitle
- BBP-functionalized biomimetic nanofibrous scaffolds can capture BMP2 and promote osteogenic differentiation
- Creators
- Qingqing Yao - University of South DakotaEric S. Sandhurst - University of South DakotaYangxi Liu - University of South DakotaHongli Sun - University of South Dakota
- Resource Type
- Journal article
- Publication Details
- Journal of materials chemistry. B, Materials for biology and medicine, Vol.5(26), pp.5196-5205
- DOI
- 10.1039/C7TB00744B
- PMID
- 29250330
- PMCID
- PMC5730084
- NLM abbreviation
- J Mater Chem B
- ISSN
- 2050-750X
- eISSN
- 2050-7518
- Language
- English
- Date published
- 2017
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Craniofacial Anomalies Research Center; Oral and Maxillofacial Surgery
- Record Identifier
- 9984367753502771
Metrics
16 Record Views