Journal article
BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction
American journal of physiology. Renal physiology, Vol.292(2), pp.F513-F522
02/01/2007
DOI: 10.1152/ajprenal.00222.2006
PMID: 17062846
Abstract
Blood oxygen level-dependent (BOLD) magnetic resonance imaging (MRI) uses deoxyhemoglobin as an endogenous contrast agent for the noninvasive assessment of tissue oxygen bioavailability. We hypothesized that intrarenal oxygenation was impaired in patients with chronic allograft nephropathy (CAN). Ten kidney-transplant recipients with CAN and nine healthy volunteers underwent BOLD-MRI. Medullary R2* (MR2*) and cortical R2* (CR2*) levels (measures directly proportional to tissue deoxyhemoglobin levels) were determined alongside urine and serum markers of oxidative stress (OS): hydrogen peroxide (H(2)O(2)), F(2)-isoprostanes, total nitric oxide (NO), heat shock protein 27 (HSP27), and total antioxidant property (TAOP). Mean MR2* and CR2* levels were significantly decreased in CAN (increased local oxyhemoglobin concentration) compared with healthy volunteers (20.7 +/- 1.6 vs. 23.1 +/- 1.8/s, P = 0.03 and 15.9 +/- 1.9 vs. 13.6 +/- 2.3/s, P = 0.05, respectively). There was a significant increase in serum and urine levels of H(2)O(2) and serum HSP27 levels in patients with CAN. Conversely, urine NO levels and TAOP were significantly increased in healthy volunteers. Multiple linear regression analyses showed a significant association between MR2* and CR2* levels and serum/urine biomarkers of OS. BOLD-MRI demonstrated significant changes in medullary and cortical oxygen bioavailability in allografts with CAN. These correlated with serum/urine biomarkers of OS, suggesting an association between intrarenal oxygenation and OS.
Details
- Title: Subtitle
- BOLD-MRI assessment of intrarenal oxygenation and oxidative stress in patients with chronic kidney allograft dysfunction
- Creators
- Arjang Djamali - University of Wisconsin–MadisonElizabeth A Sadowski - University of Wisconsin–MadisonRebecca J Muehrer - University of Wisconsin–MadisonShannon Reese - University of Wisconsin–MadisonChanigan Smavatkul - University of Wisconsin–MadisonAparna Vidyasagar - University of Wisconsin–MadisonSean B Fain - University of Wisconsin–MadisonRyan C Lipscomb - University of Wisconsin–MadisonDebra H Hullett - University of Wisconsin–MadisonMillie Samaniego-Picota - Internal Medicine - NephrologyThomas M Grist - University of Wisconsin–MadisonBryan N Becker - University of Wisconsin–Madison
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Renal physiology, Vol.292(2), pp.F513-F522
- DOI
- 10.1152/ajprenal.00222.2006
- PMID
- 17062846
- ISSN
- 1931-857X
- eISSN
- 1522-1466
- Language
- English
- Date published
- 02/01/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Electrical and Computer Engineering; Health and Human Physiology
- Record Identifier
- 9984274955202771
Metrics
77 Record Views