BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1)

Philip H Jones; Martina Maric; Marisa N Madison; Wendy Maury; Richard J Roller; Chioma M Okeoma

doi:10.1016/j.virol.2013.01.010

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BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1)

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BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1)

Philip H Jones, Martina Maric, Marisa N Madison, Wendy Maury, Richard J Roller and Chioma M Okeoma

Virology (New York, N.Y.), Vol.438(1), pp.37-49

03/30/2013

DOI: 10.1016/j.virol.2013.01.010

PMCID: PMC4086190

PMID: 23411007

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Abstract

Chikungunya virus (CHIKV) is a re-emerging alphavirus transmitted by Aedes mosquitoes. Infection with CHIKV elicits a type I interferon response that facilities virus clearance, probably through the action of down-stream effectors such as antiviral IFN-stimulated genes (ISGs). Bone marrow stromal antigen 2 (BST-2) is an ISG shown to restrict HIV-1 replication by preventing the infection of bystander cells by tethering progeny virions on the surface of infected cells. Here we show that enrichment of cell surface BST-2 results in retention of CHIKV virus like particles (VLPs) on the cell membrane. BST-2 was found to co-localize with CHIKV structural protein E1 in the context of VLPs without any noticeable effect on BST-2 level. However, CHIKV nonstructural protein 1 (nsP1) overcomes BST-2-mediated VLPs tethering by down-regulating BST-2 expression. We conclude that BST-2 tethers CHIKV VLPs on the host cell plasma membrane and identify CHIKV nsP1 as a novel BST-2 antagonist. [Display omitted] ► BST-2/tetherin retains CHIKV VLPs on the cell membrane. ► CHIKV structural (E1) and nonstructural proteins (nsP1) localizes with BST-2. ► BST-2 is in complex with E1 and nsP1. ► nsP1 but not E1 counteracts BST-2-mediated tethering of CHIKV VLPs. ► nsP1 but not E1 down-regulates BST-2 expression.

BST-2

Tetherin

nsP1

SEM

VLP

CHIKV

Nonstructural protein

Details

Title: Subtitle: BST-2/tetherin-mediated restriction of chikungunya (CHIKV) VLP budding is counteracted by CHIKV non-structural protein 1 (nsP1)
Creators: Philip H Jones - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Martina Maric - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Marisa N Madison - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Wendy Maury - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Richard J Roller - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Chioma M Okeoma - Department of Microbiology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.438(1), pp.37-49
DOI: 10.1016/j.virol.2013.01.010
PMID: 23411007
PMCID: PMC4086190
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Grant note: name: Departmental Support; name: Immunology T32 Training Grant
Language: English
Date published: 03/30/2013
Academic Unit: Microbiology and Immunology
Record Identifier: 9984001150702771

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