Journal article
Backbone and side-chain NMR assignments for the C-terminal domain of mammalian Vps28
Biomolecular NMR assignments, Vol.9(1), pp.21-24
04/01/2015
DOI: 10.1007/s12104-013-9537-8
PMCID: PMC4470380
PMID: 24366722
Abstract
Vps28 is one of four cytosolic proteins comprising the endosomal sorting complex required for transport I (ESCRT-I). ESCRT-I is involved in sorting ubiquitinated proteins to multivesicular bodies as well as in mediating budding of retroviruses. Here, we report the backbone and side-chain assignments of the mammalian C-terminal domain of Vps28 (mVps28(CTD)), which is involved in interactions with other ESCRT components. We also compare the predicted secondary structures of mVps28(CTD) with those of the published X-ray crystal structures of Saccharomyces cerevisiae and Xenopus laevis Vps28(CTD). These NMR resonance assignments will facilitate chemical shift mapping and structural determination of mammalian Vps28 interactions with other components of the endosomal sorting machinery that sorts ubiquitinated proteins for lysosomal degradation.
Details
- Title: Subtitle
- Backbone and side-chain NMR assignments for the C-terminal domain of mammalian Vps28
- Creators
- Tabitha A. Peterson - University of IowaLiping Yu - University of IowaRobert C. Piper - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Biomolecular NMR assignments, Vol.9(1), pp.21-24
- DOI
- 10.1007/s12104-013-9537-8
- PMID
- 24366722
- PMCID
- PMC4470380
- NLM abbreviation
- Biomol NMR Assign
- ISSN
- 1874-2718
- eISSN
- 1874-270X
- Publisher
- Springer Nature
- Number of pages
- 4
- Grant note
- R01GM058202 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 5R01GM058202 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 04/01/2015
- Academic Unit
- Molecular Physiology and Biophysics; Biochemistry and Molecular Biology; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297495302771
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