Journal article
Bacterial Scavengase p20 Is Structurally and Functionally Related to Peroxiredoxins
Biochemical and biophysical research communications, Vol.233(3), pp.848-852
04/28/1997
DOI: 10.1006/bbrc.1997.6564
PMID: 9168946
Abstract
Scavengase p20 was recently identified as a novel family of bacterial antioxidant enzymes possessing thioredoxin-linked thiol peroxidase activity. In this study, theEscherichia coligene coding for scavengase p20 was isolated from three different strains and the nucleotide sequence was determined. Multiple alignment of amino acid sequence revealed that a previously unidentified Cys-61 is most conserved among all bacterial p20 scavengases and corresponds to the active site in the well-characterized peroxiredoxins. Phylogenetic analysis further supported that scavengase p20 is a novel subfamily of peroxiredoxins. Site-directed mutagenesis studies demonstrated that Cys-61 is indispensable for the antioxidant activities of scavengase p20. Taken together, our findings strongly suggest that the p20 scavengases are structurally and functionally related to peroxiredoxins.
Details
- Title: Subtitle
- Bacterial Scavengase p20 Is Structurally and Functionally Related to Peroxiredoxins
- Creators
- Yuan Zhou - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of ChinaXiao-Yu Wan - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of ChinaHai-Lin Wang - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of ChinaZi-Ying Yan - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of ChinaYun De Hou - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of ChinaDong-Yan Jin - National Key Laboratory of Molecular Biology and Genetic Engineering, Chinese Academy of Preventive Medicine, 100 Yingxin Street, Beijing, 100052, People's Republic of China
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.233(3), pp.848-852
- DOI
- 10.1006/bbrc.1997.6564
- PMID
- 9168946
- NLM abbreviation
- Biochem Biophys Res Commun
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 04/28/1997
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284341302771
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