Journal article
Bacterial adhesion and growth on a polymer brush-coating
Biomaterials, Vol.29(30), pp.4117-4121
10/01/2008
DOI: 10.1016/j.biomaterials.2008.07.014
PMID: 18674814
Abstract
Biomaterials-related infections pose serious problems in implant surgery, despite the development of non-adhesive coatings. Non-adhesive coatings, like polymer brush-coatings, have so far only been investigated with respect to preventing initial bacterial adhesion, but never with respect to effects on kinetics of bacterial growth. Here, we compare adhesion and 20
h growth of three bacterial strains (
Staphylococcus aureus,
Staphylococcus epidermidis and
Pseudomonas aeruginosa) on pristine and brush-coated silicone rubber in a parallel plate flow chamber. Brush-coatings were made using a tri-block copolymer of polyethylene oxide (PEO) and polypropylene oxide (PPO). Brush-coatings prevented adhesion of staphylococci to below 5
×
10
5
cm
−2 after 30
min, which is a 10-fold reduction compared to pristine silicone rubber. Biofilms grew on both brush-coated and pristine silicone rubber, while the viability of biofilms on brush-coatings was higher than on pristine silicone rubber. However, biofilms on brush-coatings developed more slowly and detached almost fully by high fluid shear. Brush-coating remained non-adhesive after
S. epidermidis biofilm formation and subsequent removal whereas a part of its functionality was lost after removal of
S. aureus biofilms. Adhesion, growth and detachment of
P. aeruginosa were not significantly different on brush-coatings as compared with pristine silicone rubber, although here too the viability of biofilms on brush-coatings was higher. We conclude that polymer brush-coatings strongly reduce initial adhesion of staphylococci and delay their biofilm growth. In addition, biofilms on brush-coatings are more viable and easily removed by the application of fluid shear.
Details
- Title: Subtitle
- Bacterial adhesion and growth on a polymer brush-coating
- Creators
- M. Reza Nejadnik - University Medical Center GroningenHenny C Van der Mei - University Medical Center GroningenWillem Norde - University Medical Center GroningenHenk J. Busscher - University Medical Center Groningen
- Resource Type
- Journal article
- Publication Details
- Biomaterials, Vol.29(30), pp.4117-4121
- DOI
- 10.1016/j.biomaterials.2008.07.014
- PMID
- 18674814
- NLM abbreviation
- Biomaterials
- ISSN
- 0142-9612
- eISSN
- 1878-5905
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 10/01/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420935002771
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