Bacterial biogeography of adult airways in atopic asthma

Juliana Durack; Yvonne J. Huang; Snehal Nariya; Laura S. Christian; K Mark Ansel; Avraham Beigelman; Mario Castro; Anne-Marie Dyer; Elliot Israel; Monica Kraft; Richard J. Martin; David T. Mauger; Sharon R. Rosenberg; Tonya S. King; Steven R. White; Loren C. Denlinger; Fernando Holguin; Stephen C. Lazarus; Njira Lugogo; Stephen P. Peters; Lewis J. Smith; Michael E. Wechsler; Susan V. Lynch; Homer A. Boushey; National Heart, Lung and Blood Institute’s “AsthmaNet”

doi:10.1186/s40168-018-0487-3

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Bacterial biogeography of adult airways in atopic asthma

Journal article

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Peer reviewed

Bacterial biogeography of adult airways in atopic asthma

Juliana Durack, Yvonne J. Huang, Snehal Nariya, Laura S. Christian, K Mark Ansel, Avraham Beigelman, Mario Castro, Anne-Marie Dyer, Elliot Israel, Monica Kraft, …

Microbiome, Vol.6(1), pp.104-104

06/09/2018

DOI: 10.1186/s40168-018-0487-3

PMCID: PMC5994066

PMID: 29885665

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Abstract

Background Perturbations to the composition and function of bronchial bacterial communities appear to contribute to the pathophysiology of asthma. Unraveling the nature and mechanisms of these complex associations will require large longitudinal studies, for which bronchoscopy is poorly suited. Studies of samples obtained by sputum induction and nasopharyngeal brushing or lavage have also reported asthma-associated microbiota characteristics. It remains unknown, however, whether the microbiota detected in these less-invasive sample types reflect the composition of bronchial microbiota in asthma. Results Bacterial microbiota in paired protected bronchial brushings (BB; n = 45), induced sputum (IS; n = 45), oral wash (OW; n = 45), and nasal brushings (NB; n = 27) from adults with mild atopic asthma (AA), atopy without asthma (ANA), and healthy controls (HC) were profiled using 16S rRNA gene sequencing. Though microbiota composition varied with sample type (p < 0.001), compositional similarity was greatest for BB-IS, particularly in AAs and ANAs. The abundance of genera detected in BB correlated with those detected in IS and OW (r median [IQR] 0.869 [0.748–0.942] and 0.822 [0.687–0.909] respectively), but not with those in NB (r = 0.004 [− 0.003–0.011]). The number of taxa shared between IS-BB and NB-BB was greater in AAs than in HCs (p < 0.05) and included taxa previously associated with asthma. Of the genera abundant in NB, only Moraxella correlated positively with abundance in BB; specific members of this genus were shared between the two compartments only in AAs. Relative abundance of Moraxella in NB of AAs correlated negatively with that of Corynebacterium but positively with markers of eosinophilic inflammation in the blood and BAL fluid. The genus, Corynebacterium, trended to dominate all NB samples of HCs but only half of AAs (p = 0.07), in whom abundance of this genus was negatively associated with markers of eosinophilic inflammation. Conclusions Induced sputum is superior to nasal brush or oral wash for assessing bronchial microbiota composition in asthmatic adults. Although compositionally similar to the bronchial microbiota, the microbiota in induced sputum are distinct, reflecting enrichment of oral bacteria. Specific bacterial genera are shared between the nasal and the bronchial mucosa which are associated with markers of systemic and bronchial inflammation.

Adult asthma

Atopy

Bronchial microbiota

Corynebacterium

Eosinophilic inflammation

Induced sputum microbiota

Lower airways

Moraxella

Nasal microbiota

Oral microbiota

Upper airways

Details

Title: Subtitle: Bacterial biogeography of adult airways in atopic asthma
Creators: Juliana Durack - University of California, San Francisco
Yvonne J. Huang - University of Michigan
Snehal Nariya - University of California, San Francisco
Laura S. Christian - University of California, San Francisco
K Mark Ansel - San Francisco, CA USA
Avraham Beigelman - Washington University in St. Louis
Mario Castro - Washington University in St. Louis
Anne-Marie Dyer - Pennsylvania State University
Elliot Israel - Brigham and Women's Hospital
Monica Kraft - University of Arizona
Richard J. Martin - Jewish Hospital
David T. Mauger - Pennsylvania State University
Sharon R. Rosenberg - Northwestern University
Tonya S. King - Pennsylvania State University
Steven R. White - University of Chicago
Loren C. Denlinger - University of Wisconsin–Madison
Fernando Holguin - University of Pittsburgh
Stephen C. Lazarus - University of California, San Francisco
Njira Lugogo - Duke University
Stephen P. Peters - Wake Forest University
Lewis J. Smith - Northwestern University
Michael E. Wechsler - Jewish Hospital
Susan V. Lynch - University of California, San Francisco
Homer A. Boushey - University of California, San Francisco
National Heart, Lung and Blood Institute’s “AsthmaNet”
Contributors: Vanessa Curtis (Contributor) - University of Iowa, Endocrinology and Diabetes
Resource Type: Journal article
Publication Details: Microbiome, Vol.6(1), pp.104-104
DOI: 10.1186/s40168-018-0487-3
PMID: 29885665
PMCID: PMC5994066
NLM abbreviation: Microbiome
ISSN: 2049-2618
eISSN: 2049-2618
Publisher: BioMed Central
Grant note: HL098107 / ; AI114271 / ;
Language: English
Date published: 06/09/2018
Academic Unit: Endocrinology and Diabetes; Stead Family Department of Pediatrics
Record Identifier: 9984354157802771

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