Journal article
Bacterial colonization of polymer brush-coated and pristine silicone rubber implanted in infected pockets in mice
Journal of antimicrobial chemotherapy, Vol.62(6), pp.1323-1325
12/01/2008
DOI: 10.1093/jac/dkn395
PMID: 18812426
Abstract
Objectives Curing biomaterial-associated infection (BAI) frequently includes antibiotic treatment, implant removal and re-implantation. However, revision implants are at a greater risk of infection as they may attract bacteria from their infected surroundings. Polymer brush-coatings attract low numbers of bacteria, but the virtue of polymer brush-coatings in vivo has seldom been investigated. Here, we determine the possible benefits of polymer brush-coated versus pristine silicone rubber in revision surgery, using a murine model. Methods BAI was induced in 26 mice by subcutaneous implantation of silicone rubber discs with a biofilm of Staphylococcus aureus Xen29. During the development of BAI, half of the mice received rifampicin/vancomycin treatment. After 5 days, the infected discs were removed from all mice, and either a polymer brush-coated or pristine silicone rubber disc was re-implanted. Revision discs were explanted after 5 days, and the number of cfu cultured from the discs and the surrounding tissue was determined. Results None of the polymer brush-coated discs after antibiotic treatment appeared colonized by staphylococci, whereas 83% of the pristine silicone rubber discs were re-infected. Polymer brush-coated discs also showed reduced colonization rates in the absence of antibiotic treatment when compared with pristine silicone rubber discs. Tissue surrounding the discs was culture-positive in all cases. Conclusions Polymer brush-coatings are less prone to re-infection than pristine silicone rubber when used in revision surgery, i.e. when implanted in a subcutaneous pocket infected by a staphylococcal BAI. Antibiotic pre-treatment during the development of BAI hardly had any effect in preventing the colonization of pristine silicone rubber.
Details
- Title: Subtitle
- Bacterial colonization of polymer brush-coated and pristine silicone rubber implanted in infected pockets in mice
- Creators
- M. Reza Nejadnik - University Medical Center GroningenAnton F. Engelsman - University Medical Center GroningenIsabel C. Saldarriaga Fernandez - University Medical Center GroningenHenk J. Busscher - University Medical Center GroningenWillem Norde - University Medical Center GroningenHenny C. van der Mei - University Medical Center Groningen
- Resource Type
- Journal article
- Publication Details
- Journal of antimicrobial chemotherapy, Vol.62(6), pp.1323-1325
- Publisher
- Oxford University Press
- DOI
- 10.1093/jac/dkn395
- PMID
- 18812426
- ISSN
- 0305-7453
- eISSN
- 1460-2091
- Language
- English
- Date published
- 12/01/2008
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
- Record Identifier
- 9984420837502771
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