Journal article
Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78
Applied microbiology and biotechnology, Vol.106(5-6), pp.2235-2248
03/01/2022
DOI: 10.1007/s00253-022-11815-3
PMCID: PMC8930801
PMID: 35166894
Abstract
As large-scale outdoor production cannot be done in complete containment, cultures are (more) open for bacteria, which may affect the productivity and stability of the algae production process. We investigated the bacterial diversity in two indoor reactors and four pilot-scale outdoor reactors for the production of
Nannochloropsis
sp. CCAP211/78 spanning four months of operation from July to October. Illumina sequencing of 16S rRNA gene amplicons demonstrated that a wide variety of bacteria were present in all reactor types, with predominance of
Bacteroidetes
and
Alphaproteobacteria
. Bacterial communities were significantly different between all reactor types (except between the horizontal tubular reactor and the vertical tubular reactor) and also between runs in each reactor. Bacteria common to the majority of samples included one member of the
Saprospiraceae
family and one of the
NS11-12_marine group
(both
Bacteroidetes
). Hierarchical clustering analysis revealed two phases during the cultivation period separated by a major shift in bacterial community composition in the horizontal tubular reactor, the vertical tubular reactor and the raceway pond with a strong decrease of the
Saprospiraceae
and
NS11-12_marine group
that initially dominated the bacterial communities. Furthermore, we observed a less consistent pattern of bacterial taxa appearing in different reactors and runs, most of which belonging to the classes
Deltaproteobacteria
and
Flavobacteriia
. In addition, canonical correspondence analysis showed that the bacterial community composition was significantly correlated with the nitrate concentration. This study contributes to our understanding of bacterial diversity and composition in different types of outdoor reactors exposed to a range of dynamic biotic and abiotic factors.
Key points
•
Reactor types had significantly different bacterial communities except HT and VT
•
The inoculum source and physiochemical factors together affect bacterial community
•
The bacterial family Saprospiraceae is positively correlated to microalgal growth
Details
- Title: Subtitle
- Bacterial diversity in different outdoor pilot plant photobioreactor types during production of the microalga Nannochloropsis sp. CCAP211/78
- Creators
- Jie Lian - Wageningen University & ResearchGeorg Steinert - Wageningen University & ResearchJeroen de Vree - Wageningen University & ResearchSven Meijer - Wageningen University & ResearchChrista Heryanto - Wageningen University & ResearchRouke Bosma - Wageningen University & ResearchRené H. Wijffels - Wageningen University & ResearchMaria J. Barbosa - PO Box 16, 6700 AA Wageningen, The NetherlandsHauke Smidt - Wageningen University & ResearchDetmer Sipkema - Wageningen University & Research
- Resource Type
- Journal article
- Publication Details
- Applied microbiology and biotechnology, Vol.106(5-6), pp.2235-2248
- DOI
- 10.1007/s00253-022-11815-3
- PMID
- 35166894
- PMCID
- PMC8930801
- NLM abbreviation
- Appl Microbiol Biotechnol
- ISSN
- 0175-7598
- eISSN
- 1432-0614
- Publisher
- Springer Berlin Heidelberg
- Language
- English
- Date published
- 03/01/2022
- Academic Unit
- International Programs; Spanish and Portuguese
- Record Identifier
- 9984398035202771
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