Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

Yanhe Ma; Weizhou Zhang; Yanfen Xue; Peijin Zhou; Antonio Ventosa; William Grant

doi:10.1007/s00792-003-0358-z

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Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

Journal article

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Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

Yanhe Ma, Weizhou Zhang, Yanfen Xue, Peijin Zhou, Antonio Ventosa and William Grant

Extremophiles : life under extreme conditions, Vol.8(1), pp.45-51

02/2004

DOI: 10.1007/s00792-003-0358-z

PMID: 15064989

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Abstract

Bacterial diversity associated with Baer Soda Lake in Inner Mongolia of China was investigated using a culture-independent method. Bacterial 16S rRNA gene libraries were generated using bacterial oligonucleotide primers, and 16S rRNA gene sequences of 58 clones were analyzed phylogenetically. The library was dominated by 16S rDNAs of Gram-negative bacteria (24% α-Proteobacteria, 31% β-Proteobacteria, 33% γ-Proteobacteria, and 2% δ-Proteobacteria), with a lower percentage of clones corresponding to Gram-positive bacteria. Forty cloned sequences were similar to that of known bacterial isolates (>97% sequence similarity), represented by the species of the genera Brevundimonas, Comamonas, Alcaligenes, Stenotrophomonas, and Klebsiella. Eighteen cloned sequences showed less affiliation with known taxa (<97% sequence similarity) and may represent novel taxa.

Phylogenetic analysis

Alkaliphilic bacteria

Soda lake

LifeSciences

Biodiversity

16S rRNA gene

Details

Title: Subtitle: Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses
Creators: Yanhe Ma - Department of Microbial Physiology and Ecology, Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China
Weizhou Zhang - Department of Microbial Physiology and Ecology, Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China
Yanfen Xue - Department of Microbial Physiology and Ecology, Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China
Peijin Zhou - Department of Microbial Physiology and Ecology, Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China
Antonio Ventosa - Department of Microbiology and Parasitology, Faculty of Pharmacy University of Sevilla 41012 Sevilla Spain
William Grant - Department of Microbiology and Immunology University of Leceister Leceister LE1 9HN UK
Resource Type: Journal article
Publication Details: Extremophiles : life under extreme conditions, Vol.8(1), pp.45-51
Publisher: Springer-Verlag
DOI: 10.1007/s00792-003-0358-z
PMID: 15064989
ISSN: 1431-0651
eISSN: 1433-4909
Language: English
Date published: 02/2004
Academic Unit: Radiation Oncology
Record Identifier: 9984083285202771

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