Journal article
Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels
Methods in molecular biology (Clifton, N.J.), Vol.518, pp.113-122
2009
DOI: 10.1007/978-1-59745-202-1_9
PMCID: PMC3071032
PMID: 19085128
Abstract
Bacteriophage phiC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the phiC31 integrase approach to make transgenic Xenopus laevis embryos.
Details
- Title: Subtitle
- Bacteriophage phiC31 integrase mediated transgenesis in Xenopus laevis for protein expression at endogenous levels
- Creators
- Bryan G Allen - Department of Biochemistry, University of Iowa, Iowa City, IA, USADaniel L Weeks
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.518, pp.113-122
- Publisher
- United States
- DOI
- 10.1007/978-1-59745-202-1_9
- PMID
- 19085128
- PMCID
- PMC3071032
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Grant note
- R01 GM069944 / NIGMS NIH HHS GM069944 / NIGMS NIH HHS DC007481 / NIDCD NIH HHS R01 DC007481 / NIDCD NIH HHS R01 GM069944-05A2 / NIGMS NIH HHS T32 GM007337 / NIGMS NIH HHS
- Language
- English
- Date published
- 2009
- Academic Unit
- Stead Family Department of Pediatrics; Radiation Oncology; Biochemistry and Molecular Biology
- Record Identifier
- 9984025253702771
Metrics
22 Record Views