Journal article
Basis for the Failure of Francisella tularensis Lipopolysaccharide To Prime Human Polymorphonuclear Leukocytes
Infection and immunity, Vol.74(6), pp.3277-3284
06/2006
DOI: 10.1128/IAI.02011-05
PMCID: PMC1479269
PMID: 16714555
Abstract
Francisella tularensis
is the intracellular gram-negative coccobacillus that causes tularemia, and its virulence and infectiousness make it a potential agent of bioterrorism. Previous studies using mononuclear leukocytes have shown that the lipopolysaccharide (LPS) of
F. tularensis
is neither a typical proinflammatory endotoxin nor an endotoxin antagonist. This inertness suggests that
F. tularensis
LPS does not bind host LPS-sensing molecules such as LPS-binding protein (LBP). Using priming of the polymorphonuclear leukocyte (PMN) oxidase as a measure of endotoxicity, we found that
F. tularensis
live vaccine strain LPS did not behave like either a classic endotoxin or an endotoxin antagonist in human PMNs, even when the concentration of LBP was limiting. Furthermore,
F. tularensis
LPS did not compete with a radiolabeled lipooligosaccharide from
Neisseria meningitidis
for binding to LBP or to the closely related PMN granule protein, bactericidal/permeability-increasing protein. Our results suggest that the inertness of
F. tularensis
LPS and the resistance of
F. tularensis
to oxygen-independent PMN killing may result from the inability of
F. tularensis
LPS to be recognized by these important LPS-sensing molecules of the innate immune system.
Details
- Title: Subtitle
- Basis for the Failure of Francisella tularensis Lipopolysaccharide To Prime Human Polymorphonuclear Leukocytes
- Creators
- Jason H Barker - Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical CenterJerrold Weiss - Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical CenterMichael A Apicella - Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical CenterWilliam M Nauseef - Inflammation Program and Department of Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa and Veterans Administration Medical Center
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.74(6), pp.3277-3284
- DOI
- 10.1128/IAI.02011-05
- PMID
- 16714555
- PMCID
- PMC1479269
- NLM abbreviation
- Infect Immun
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 06/2006
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094594802771
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