Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel

John D McCann; Michael J Welsh

doi:10.1152/ajplung.1990.258.6.L343

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Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel

Journal article

Peer reviewed

Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel

John D McCann and Michael J Welsh

The American journal of physiology, Vol.258(6 Pt 1), pp.L343-L348

06/1990

DOI: 10.1152/ajplung.1990.258.6.L343

PMID: 1694405

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Abstract

We previously described a Ca2(+)-activated K+ channel (KCLIC) in airway epithelial cells [J. D. McCann, J. Matsuda, M. Garcia, G. Kaczorowski, and M. J. Welsh. Am. J. Physiol 258 (Lung Cell. Mol. Physiol. 2): L334-L342, 1990]. To determine whether the KCLIC channel is a basolateral membrane channel and to understand its role in Cl- secretion, we studied airway epithelial cells grown on permeable supports. When cells were stimulated with A23187, charybdotoxin (ChTX) inhibited Cl- secretion and 86Rb efflux at the same concentrations, indicating that the KCLIC channel is required for Ca2(+)-stimulated Cl- secretion. We also investigated the function of K+ channels in adenosine 3',5'-cyclic monophosphate-stimulated secretion. Addition of isoproterenol caused a biphasic increase in Cl- secretion; the time course of the transient component correlated with the time course of the isoproterenol-induced increase in Ca2+ concentration [( Ca2+]c). ChTX inhibited the transient component, but not the prolonged component of secretion; Ba2+ inhibited the sustained component. These results suggest that when cells are grown on permeable supports isoproterenol-induced secretion depends on activation of two types of K+ channel: the KCLIC channel that is stimulated initially and a ChTX-insensitive K+ channel that is stimulated during sustained secretion. This conclusion was supported by measurement of 86Rb efflux from cell monolayers.

Membrane Potentials - drug effects

Epithelium - drug effects

Cells, Cultured

Potassium Channels - physiology

Electric Conductivity

Tetraethylammonium

Barium - pharmacology

Cell Membrane - physiology

Potassium Channels - drug effects

Calcimycin - pharmacology

Tetraethylammonium Compounds - pharmacology

Scorpion Venoms - pharmacology

Animals

Charybdotoxin

Chlorides - metabolism

Isoproterenol - pharmacology

Dogs

Trachea - physiology

Muscle, Smooth - physiology

Epithelium - physiology

Kinetics

Rubidium - metabolism

Cell Membrane - drug effects

Rubidium Radioisotopes

Details

Title: Subtitle: Basolateral K+ channels in airway epithelia. II. Role in Cl- secretion and evidence for two types of K+ channel
Creators: John D McCann - Howard Hughes Medical Institute, Department of Physiology, University of Iowa College of Medicine, Iowa City 52242
Michael J Welsh
Resource Type: Journal article
Publication Details: The American journal of physiology, Vol.258(6 Pt 1), pp.L343-L348
DOI: 10.1152/ajplung.1990.258.6.L343
PMID: 1694405
NLM abbreviation: Am J Physiol
ISSN: 0002-9513
eISSN: 2163-5773
Publisher: American Physiological Society; United States
Grant note: HL-29851 / NHLBI NIH HHS HL-42385 / NHLBI NIH HHS
Language: English
Date published: 06/1990
Academic Unit: Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
Record Identifier: 9984020995102771

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