Journal article
Bcl-2 and Bcl-xL in Peroxide-Resistant A549 and U87MG Cells
Toxicological sciences, Vol.42(2), pp.109-116
04/1998
DOI: 10.1093/toxsci/42.2.109
PMID: 9579023
Abstract
Overexpression of the bcl-2 and the related bcl-xL protoonco-gene proteins enhance cell survival by inhibiting apoptosis induced by many agents including oxidants. Whether these proteins contribute to survival in oxidant-resistant cells is not known. The current study assessed the expression of bcl-2 and bcl-xL proteins in human glioblastoma U87MG cells and human lung adenocar-cinoma A549 cells selected for resistance to 0, 50, 100, 200, and 400 μM H2O2 by exposure to this oxidant one time each passage for 9 months. When examined 7 to 32 days after cessation of peroxide exposure (times when peroxide resistance was maintained), bcl-2 protein levels were significantly increased in most peroxide-resistant U87MG cells. However, the increase was not dose dependent and was not accompanied by an increase in mRNA levels. A549 cells did not express significant levels of bcl-2 protein, although bcl-2 mRNA was detectable. A549 cells expressed large amounts of bcl-xL and immunohistochemistry demonstrated extensive localization of this protein around the nucleus. However, expression of this protein was not altered in peroxide-resistant lines nor was bcl-2 protein increased to a measurable level. U87MG cells also expressed bcl-xL but it was not altered in peroxide-resistant cells. Although the increased bcl-2 protein in peroxide-resistant U87MG cells may contribute to their oxidant tolerance, the lack of a dose-response relationship, the failure to induce bcl-xL protein, and the absence of any bcl-2 or bcl-xL protein induction in peroxide-resistant A549 cells suggest these genes are not primary factors in oxidant resistance.
Details
- Title: Subtitle
- Bcl-2 and Bcl-xL in Peroxide-Resistant A549 and U87MG Cells
- Creators
- Heidi K. Bojes - Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin Austin, Texas 78712-1074P. K. Suresh - Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin Austin, Texas 78712-1074Edward M. Mills - Purdue University West LafayetteDouglas R. Spitz - ‡Section of Cancer Biology, Radiation Oncology Center, Washington University School of Medicine St. Louis, Missouri 63108Julia E. Sim - ‡Section of Cancer Biology, Radiation Oncology Center, Washington University School of Medicine St. Louis, Missouri 63108James P. Kehrer - Division of Pharmacology and Toxicology, College of Pharmacy, The University of Texas at Austin Austin, Texas 78712-1074
- Resource Type
- Journal article
- Publication Details
- Toxicological sciences, Vol.42(2), pp.109-116
- DOI
- 10.1093/toxsci/42.2.109
- PMID
- 9579023
- NLM abbreviation
- Toxicol Sci
- ISSN
- 1096-6080
- eISSN
- 1096-0929
- Publisher
- Oxford University Press
- Comment
- Also available from Elseiver at https://doi.org/10.1006/toxs.1997.2416
- Language
- English
- Date published
- 04/1998
- Academic Unit
- Pathology; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984315657402771
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