Bcl-xL/Bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry

Yelena M Janumyan; Courtney G Sansam; Anuja Chattopadhyay; Ningli Cheng; Erinn L Soucie; Linda Z Penn; David Andrews; C.Michael Knudson; Elizabeth Yang

doi:10.1093/emboj/cdg533

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Bcl-xL/Bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry

Journal article

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Bcl-xL/Bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry

Yelena M Janumyan, Courtney G Sansam, Anuja Chattopadhyay, Ningli Cheng, Erinn L Soucie, Linda Z Penn, David Andrews, C.Michael Knudson and Elizabeth Yang

The EMBO journal, Vol.22(20), pp.5459-5470

10/15/2003

DOI: 10.1093/emboj/cdg533

PMCID: PMC213787

PMID: 14532118

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Abstract

Bcl-x L and Bcl-2 inhibit both apoptosis and proliferation. In investigating the relationship between these two functions of Bcl-x L and Bcl-2, an analysis of 24 Bcl-x L and Bcl-2 mutant alleles, including substitutions at residue Y28 previously reported to selectively abolish the cell cycle activity, showed that cell cycle delay and anti-apoptosis co-segregated in all cases. In determining whether Bcl-2 and Bcl-x L act in G 0 or G 1 , forward scatter and pyronin Y fluorescence measurements indicated that Bcl-2 and Bcl-x L cells arrested more effectively in G 0 than controls, and were delayed in G 0 –G 1 transition. The cell cycle effects of Bcl-2 and Bcl-x L were reversed by Bad, a molecule that counters the survival function of Bcl-2 and Bcl-x L . When control and Bcl-x L cells of equivalent size and pyronin Y fluorescence were compared, the kinetics of cell cycle entry were similar, demonstrating that the ability of Bcl-x L and Bcl-2 cells to enhance G 0 arrest contributes significantly to cell cycle delay. Our data suggest that cell cycle effects and increased survival both result from intrinsic functions of Bcl-2 and Bcl-x L .

Apoptosis

Cell Cycle

Bcl-2

Bcl-xL

BAD

Details

Title: Subtitle: Bcl-xL/Bcl-2 coordinately regulates apoptosis, cell cycle arrest and cell cycle entry
Creators: Yelena M Janumyan - Departments of
Courtney G Sansam - Departments of
Anuja Chattopadhyay - Departments of
Ningli Cheng - Departments of
Erinn L Soucie - Departments of
Linda Z Penn - Departments of
David Andrews - Departments of
C.Michael Knudson - Departments of
Elizabeth Yang - Departments of
Resource Type: Journal article
Publication Details: The EMBO journal, Vol.22(20), pp.5459-5470
Publisher: Oxford University Press; Oxford, UK
DOI: 10.1093/emboj/cdg533
PMID: 14532118
PMCID: PMC213787
ISSN: 0261-4189
eISSN: 1460-2075
Language: English
Date published: 10/15/2003
Academic Unit: Pathology; Radiation Oncology
Record Identifier: 9984047865802771

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