Journal article
Behavior of the idiotypic network in conventional immune responses: II. Affinity and heterogeneity of idiotypic and anti-idiotypic antibodies following immunization with T-independent and T-dependent antigens
Cellular immunology, Vol.144(2), pp.324-331
1992
DOI: 10.1016/0008-8749(92)90248-N
PMID: 1382869
Abstract
The relative affinity and heterogeneity of affinity of idiotypic and anti-idiotypic antibodies in mice immunized with the T-independent antigen DNP-Ficoll and the T-dependent antigen DNPHGG were measured by a plaque inhibition assay. Idiotypic plaque-forming cells (PFC) were detected by a conventional assay utilizing DNP-coated SRBC. Anti-idiotypic PFC were detected with SRBC coated with affinity-purified anti-DNP antibody of rabbit origin. It was found that both idiotypic and anti-idiotypic antibodies elicited by immunization with the T-independent antigen had lower affinity and were less heterogeneous than the corresponding antibodies originating in mice immunized with the T-dependent antigen. In addition, the affinity and heterogeneity values of the idiotypic antibodies were correlated with the affinity and heterogeneity values of the anti-idiotypic antibodies from the same mice. This finding indicates that idiotypic and anti-idiotypic antibodies mutually regulate each other, thus pointing to internal immunoregulatory effects of the idiotypic network with respect to these parameters.
Details
- Title: Subtitle
- Behavior of the idiotypic network in conventional immune responses: II. Affinity and heterogeneity of idiotypic and anti-idiotypic antibodies following immunization with T-independent and T-dependent antigens
- Creators
- Mariangela SegreRonald M WeigelAnnette J SchlueterDiego Segre
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.144(2), pp.324-331
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0008-8749(92)90248-N
- PMID
- 1382869
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 1992
- Academic Unit
- Molecular Physiology and Biophysics; Anatomy and Cell Biology; Pathology; Surgery; Biochemistry and Molecular Biology
- Record Identifier
- 9984047738902771
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