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Benzo[a]pyrene degradation by Sphingomonas yanoikuyae JAR02
Journal article   Peer reviewed

Benzo[a]pyrene degradation by Sphingomonas yanoikuyae JAR02

Jeremy A Rentz, Pedro J J Alvarez and Jerald L Schnoor
Environmental pollution (1987), Vol.151(3), pp.669-677
02/2008
DOI: 10.1016/j.envpol.2007.02.018
PMID: 17482734

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Abstract

Batch experiments were conducted to characterize the degradation of benzo[a]pyrene, a representative high molecular weight (HMW) polycyclic aromatic hydrocarbon (PAH), by Sphingomonas yanoikuyae JAR02. Concentrations up to the solubility limit (1.2 microg l(-1)) of benzo[a]pyrene were completely removed from solution within 20 h when the bacterium was grown on salicylate. Additional experiments with [(14)C]7-benzo[a]pyrene demonstrated 3.8% mineralization over 7 days when salicylate was present is solution, and one major radio-labeled metabolite was observed that accounted for approximately 10% of the initial radio-label. Further characterization of the radio-labeled metabolite using HPLC/MS and HPLC/MS/MS identified radio-labeled pyrene-8-hydroxy-7-carboxylic acid and unlabeled pyrene-7-hydroxy-8-carboxylic acid as novel ring-cleavage metabolites, and a benzo[a]pyrene degradation pathway was proposed. Results indicate that biostimulation of HMW PAH degradation by salicylate, a water-soluble, non-toxic substrate, has significant potential for in situ bioremediation.
Bioreactors Mass Spectrometry Biodegradation, Environmental Soil Microbiology Chromatography, High Pressure Liquid Carbon Radioisotopes - analysis Soil Pollutants - analysis Benzo(a)pyrene - analysis Soil Pollutants - metabolism Ecology - methods Carbon Radioisotopes - metabolism Salicylates - metabolism Benzo(a)pyrene - metabolism Sphingomonas - metabolism

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