Journal article
Bidirectional Transport by Distinct Populations of COPI-Coated Vesicles
Cell, Vol.90(2), pp.335-349
1997
DOI: 10.1016/S0092-8674(00)80341-4
PMID: 9244307
Abstract
Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.
Details
- Title: Subtitle
- Bidirectional Transport by Distinct Populations of COPI-Coated Vesicles
- Creators
- Lelio Orci - University of GenevaMark Stamnes - Memorial Sloan Kettering Cancer CenterMariella Ravazzola - University of GenevaMylène Amherdt - University of GenevaAlain Perrelet - University of GenevaThomas H Söllner - Memorial Sloan Kettering Cancer CenterJames E Rothman - Memorial Sloan Kettering Cancer Center
- Resource Type
- Journal article
- Publication Details
- Cell, Vol.90(2), pp.335-349
- DOI
- 10.1016/S0092-8674(00)80341-4
- PMID
- 9244307
- NLM abbreviation
- Cell
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1997
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297602402771
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