Journal article
Bifidobacterium longum Subspecies infantis Strain EVC001 Decreases Neonatal Murine Necrotizing Enterocolitis
Nutrients, Vol.14(3), p.495
01/24/2022
DOI: 10.3390/nu14030495
PMCID: PMC8839161
PMID: 35276854
Abstract
Necrotizing enterocolitis (NEC) is a disease mainly of preterm infants with a 30-50% mortality rate and long-term morbidities for survivors. Treatment strategies are limited and have not improved in decades, prompting research into prevention strategies, particularly with probiotics. Recent work with the probiotic B. infantis EVC001 suggests that this organism may generate a more appropriate microbiome for preterm infants who generally have inappropriate gut colonization and inflammation, both risk factors for NEC. Experimental NEC involving Paneth cell disruption in combination with bacterial dysbiosis or formula feeding was induced in P14-16 C57Bl/6 mice with or without gavaged B. infantis. Following completion of the model, serum, small intestinal tissue, the cecum, and colon were harvested to examine inflammatory cytokines, injury, and the microbiome, respectively. EVC001 treatment significantly decreased NEC in a bacterial dysbiosis dependent model, but this decrease was model-dependent. In the NEC model dependent on formula feeding, no difference in injury was observed, but trending to significant differences was observed in serum cytokines. EVC001 also improved wound closure at six and twelve hours compared to the sham control in intestinal epithelial monolayers. These findings suggest that B. infantis EVC001 can prevent experimental NEC through anti-inflammatory and epithelial barrier restoration properties.
Details
- Title: Subtitle
- Bifidobacterium longum Subspecies infantis Strain EVC001 Decreases Neonatal Murine Necrotizing Enterocolitis
- Creators
- Shiloh R. Lueschow - University of IowaTimothy J. Boly - University of IowaSteven A. Frese - University of Nevada, RenoGiorgio Casaburi - Metabiomics, Dept Bioinformat, Carlsbad, CA 92008 USARyan D. Mitchell - Evolve Biosyst Inc, Davis, CA 95618 USABethany M. Henrick - University of Nebraska–LincolnSteven J. McElroy - University of California, Davis
- Resource Type
- Journal article
- Publication Details
- Nutrients, Vol.14(3), p.495
- DOI
- 10.3390/nu14030495
- PMID
- 35276854
- PMCID
- PMC8839161
- NLM abbreviation
- Nutrients
- ISSN
- 2072-6643
- eISSN
- 2072-6643
- Publisher
- Mdpi
- Number of pages
- 19
- Grant note
- 1S10OD010786-01 / NIH Shared Instrumentation Grant; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 01/24/2022
- Academic Unit
- Stead Family Department of Pediatrics; Neonatology
- Record Identifier
- 9984775275802771
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