Journal article
Bile and pancreatic juice replacement ameliorates early ligation-induced acute pancreatitis in rats
The American journal of surgery, Vol.169(4), pp.391-399
1995
DOI: 10.1016/S0002-9610(99)80183-4
PMID: 7535017
Abstract
In healthy rats, combined bile and pancreatic juice diversion from gut has a synergistic rather than additive effect on stimulation of exocrine pancreatic protein secretion. We hypothesized that exclusion of combined bile and pancreatic juice from gut exacerbates bile and pancreatic-duct ligation-induced acute pancreatitis in rats to a greater extent than exclusion of either bile or pancreatic juice alone.
Bile and pancreatic juice (obtained fresh from donor rats) were replaced, separately or together, via a duodenal fistula beginning immediately before 6 hours of duct ligation. Pancreatic morphologic changes were evaluated with an acute pancreatitis histology score and morphometric quantitation of acinar-cell necrosis. Plasma amylase and cholecystokinin concentrations and pancreatic subcellular distribution of cathepsin B activity were determined. Characteristics of bile and pancreatic juice obtained from donor rats were also studied.
Combined bile and pancreatic juice replacement limited the increase in acute pancreatitis histology score by 77%, acinar cell necrosis by 95%, hyperamylasemia by 77%, and hypercholecystokininemia by 99%, while preventing subcellular redistribution of cathepsin B. Amelioration of pancreatic morphologic changes was significantly greater with combined bile and pancreatic juice replacement than with replacement of either bile or pancreatic juice alone.
In this experimental corollary of early gallstone-induced acute pancreatitis, combined bile and pancreatic juice exclusion from gut contributes to disease pathogenesis to a greater extent than exclusion of either bile or pancreatic juice alone.
Details
- Title: Subtitle
- Bile and pancreatic juice replacement ameliorates early ligation-induced acute pancreatitis in rats
- Creators
- Isaac Samuel - From the Department of Surgery, Northwestern University Medical School, and Surgical and Research Services, Veterans Affairs Lakeside Medical Center, Chicago, Illinois, USAYasuo Toriumi - From the Department of Surgery, Northwestern University Medical School, and Surgical and Research Services, Veterans Affairs Lakeside Medical Center, Chicago, Illinois, USADaniel P Wilcockson - From the Department of Surgery, Northwestern University Medical School, and Surgical and Research Services, Veterans Affairs Lakeside Medical Center, Chicago, Illinois, USACharles M Turkelson - From the Department Research Service, Veterans Affairs Medical Center, Kansas City, Missouri, USATravis E Solomon - From the Department Research Service, Veterans Affairs Medical Center, Kansas City, Missouri, USARaymond J Joehl - From the Department of Surgery, Northwestern University Medical School, and Surgical and Research Services, Veterans Affairs Lakeside Medical Center, Chicago, Illinois, USA
- Resource Type
- Journal article
- Publication Details
- The American journal of surgery, Vol.169(4), pp.391-399
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0002-9610(99)80183-4
- PMID
- 7535017
- ISSN
- 0002-9610
- eISSN
- 1879-1883
- Language
- English
- Date published
- 1995
- Academic Unit
- Surgery
- Record Identifier
- 9984051500702771
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