Journal article
Bile-pancreatic juice exclusion increases p38MAPK activation and TNF-alpha production in ligation-induced acute pancreatitis in rats
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.5(1), pp.20-26
2005
DOI: 10.1159/000084486
PMID: 15775695
Abstract
Acute pancreatitis is associated with stress kinase activation and cytokine production. We hypothesize that bile-pancreatic juice exclusion activates p38(MAPK) and induces TNF-alpha production in ligation-induced acute pancreatitis. We compared rats with 1-3 h of duct ligation, duct ligation with duodenal bile-pancreatic juice replacement from a donor rat, and sham operation. Pancreatic homogenates were analyzed as follows: (a) Immunoblots using phospho-specific p38(MAPK) antibody showed increased p38(MAPK) activation after ligation that was inhibited by bile-pancreatic juice replacement. (b) Immune-complex kinase assay using ATF-2 as substrate showed increased p38(MAPK) activation after ligation that was subdued by bile-pancreatic juice replacement. (c) ELISA showed increased pancreatic TNF-alpha production after ligation that was significantly ameliorated by bile-pancreatic juice replacement.
Bile-pancreatic juice exclusion from gut increases p38(MAPK) activation and TNF-alpha production in this experimental model. Our findings support our central hypothesis that bile-pancreatic juice exclusion exacerbates cell stress and acute inflammation in ligation-induced acute pancreatitis.
Details
- Title: Subtitle
- Bile-pancreatic juice exclusion increases p38MAPK activation and TNF-alpha production in ligation-induced acute pancreatitis in rats
- Creators
- Isaac Samuel - Roy J. and Lucille A. Carver College of MedicineSmita Zaheer - Roy J. and Lucille A. Carver College of MedicineAsgar Zaheer - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.5(1), pp.20-26
- DOI
- 10.1159/000084486
- PMID
- 15775695
- ISSN
- 1424-3903
- eISSN
- 1424-3911
- Grant note
- K08-DK062805 / NIDDK NIH HHS
- Language
- English
- Date published
- 2005
- Academic Unit
- Neurology; Surgery
- Record Identifier
- 9984322808102771
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