Journal article
Binding Preferences for GPIHBP1, a Glycosylphosphatidylinositol-Anchored Protein of Capillary Endothelial Cells
Arteriosclerosis, thrombosis, and vascular biology, Vol.31(1), pp.176-182
2011
DOI: 10.1161/ATVBAHA.110.214718
PMID: 20966398
Abstract
Objective: To define the ability of GPIHBP1 to bind other lipase family members and other apolipoproteins (apos) and lipoproteins.
Methods and results: GPIHBP1, a GPI-anchored lymphocyte antigen (Ly)6 protein of capillary endothelial cells, binds lipoprotein lipase (LPL) avidly, but its ability to bind related lipase family members has never been evaluated. As judged by cell-based and cell-free binding assays, LPL binds to GPIHBP1, but other members of the lipase family do not. We also examined the binding of apoAV-phospholipid disks to GPIHBP1. ApoAV binds avidly to GPIHBP1-transfected cells; this binding requires GPIHBP1's amino-terminal acidic domain and is independent of its cysteine-rich Ly6 domain (the latter domain is essential for LPL binding). GPIHBP1-transfected cells did not bind high-density lipoprotein. Chylomicrons bind avidly to GPIHBP1-transfected Chinese hamster ovary cells, but this binding is dependent on GPIHBP1's ability to bind LPL within the cell culture medium.
Conclusions: GPIHBP1 binds LPL but does not bind other lipase family members. GPIHBP1 binds apoAV but does not bind apoAI or high-density lipoprotein. The ability of GPIHBP1-transfected Chinese hamster ovary cells to bind chylomicrons is mediated by LPL; chylomicron binding does not occur unless GPIHBP1 first captures LPL from the cell culture medium.
Details
- Title: Subtitle
- Binding Preferences for GPIHBP1, a Glycosylphosphatidylinositol-Anchored Protein of Capillary Endothelial Cells
- Creators
- Peter GIN - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United StatesAnne P BEIGNEUX - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United StatesConstance VOSS - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United StatesBrandon S. J DAVIES - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United StatesJennifer A BECKSTEAD - Children's Hospital Oakland Research Institute, Oakland, Calif, United StatesRobert O RYAN - Children's Hospital Oakland Research Institute, Oakland, Calif, United StatesAndré BENSADOUN - Division of Nutritional Science , Cornell University, Ithaca, NY, UkraineLoren G FONG - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United StatesStephen G YOUNG - Department of Medicine, David Geffen School of Medicine, University of California, Los Angeles, United States
- Resource Type
- Journal article
- Publication Details
- Arteriosclerosis, thrombosis, and vascular biology, Vol.31(1), pp.176-182
- Publisher
- Lippincott Williams & Wilkins; Philadelphia, PA
- DOI
- 10.1161/ATVBAHA.110.214718
- PMID
- 20966398
- ISSN
- 1079-5642
- eISSN
- 1524-4636
- Language
- English
- Date published
- 2011
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
- Record Identifier
- 9984025271702771
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