Journal article
Binding of toxic-shock-syndrome toxin-1 to human peripheral blood mononuclear cells
The Journal of infectious diseases, Vol.156(1), pp.122-129
07/1987
DOI: 10.1093/infdis/156.1.122
PMID: 3598213
Abstract
Toxic-shock-syndrome toxin-1 (TSST-1), produced by Staphylococcus aureus and associated with toxic shock syndrome, functions in vitro as both a lymphoproliferative and immunosuppressive protein for human peripheral blood mononuclear cells (PBMs). We analyzed TSST-1-target cell interactions by receptor-ligand binding analyses. In competitive binding experiments, 2 X 10(5) human PBMs or purified cell populations were incubated in the presence of small amounts of (5-50 ng) of 125I-labeled TSST-1 and increasing amounts of unlabeled TSST-1 (25-10,000 ng). Data were analyzed by the method of Scatchard. Toxin-specific receptors were shown to exist on T lymphocytes within the PBM population. T4+ cells had 27.5 X 10(6) receptors per cell, and T8+ cells had 9 X 10(6) receptors per cell. T4+ and T8+ receptors had dissociation constants of 2.58 X 10(-8) M and 1.8 X 10(-8) M, respectively. These studies confirm earlier work showing that TSST-1 causes the functional activation of a population of T lymphocytes involved in suppression of immunoglobulin responses.
Details
- Title: Subtitle
- Binding of toxic-shock-syndrome toxin-1 to human peripheral blood mononuclear cells
- Creators
- Nancy J Poindexter - University of MinnesotaPatrick M Schlievert - University of Minnesota
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.156(1), pp.122-129
- Publisher
- United States
- DOI
- 10.1093/infdis/156.1.122
- PMID
- 3598213
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 07/1987
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001111802771
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